Isolation and quantitation of Clostridium difficile in aqueous and fecal matter using two types of selective media

• Published in: Journal of microbiology, immunology and infection Vol. 49; no. 3; pp. 445 - 447
• Main Authors: Housman, Seth T, Banevicius, Mary Anne, Lamb, Lucinda M, Nicolau, David P
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.06.2016; Elsevier
• Subjects: Bacteriological Techniques - methods; Cefoxitin - pharmacology; Chromogenic agar; Chromogenic Compounds - pharmacology; Clostridium difficile; Clostridium difficile - growth & development; Clostridium difficile - isolation & purification; Culture Media; Cycloserine - pharmacology; Cycloserine, cefoxitin, and fructose-containing agar with horse blood and taurocholate; Enterocolitis, Pseudomembranous - diagnosis; Enterocolitis, Pseudomembranous - microbiology; Feces - microbiology; Fructose - metabolism; Humans; Infectious Disease; Medical Education; Chromogenic agar; Cycloserine, cefoxitin, and fructose-containing agar with horse blood and taurocholate; Clostridium difficile
• ISSN: 1684-1182; 1995-9133
• DOI: 10.1016/j.jmii.2015.05.012

Abstract We evaluated the isolation and quantitation of Clostridium difficile from aqueous and fecal samples utilizing ChromID CDIF and cycloserine, cefoxitin, and fructose-containing agar with horse blood and taurocholate media. Growth was similar between the two. ChromID CDIF provided enhanced isolation and required no ethanol pretreatment to inhibit normal flora. ChromID CDIF also improved turn-around time, requiring only 24 hours' incubation.