Simplified microenvironments and reduced cell culture size influence the cell differentiation outcome in cellular microarrays

• Published in: Journal of materials science. Materials in medicine Vol. 24; no. 1; pp. 189 - 198
• Main Authors: Rodríguez-Seguí, Santiago A., Ortuño, María José, Ventura, Francesc, Martínez, Elena, Samitier, Josep
• Format: Journal Article
• Language: English
• Published: Boston Springer US 2013; Springer; Springer Nature B.V
• Subjects: Animals; Biological and medical sciences; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Bone Morphogenetic Protein 2 - metabolism; Cell adhesion & migration; Cell culture; Cell Differentiation; Cellular Microenvironment; Ceramics; Chemistry and Materials Science; CHO Cells; Composites; Cricetinae; Cricetulus; Glass; Humans; Materials Science; Medical sciences; Mice; Natural Materials; Polymer Sciences; Protein Array Analysis; Regenerative Medicine/Tissue Engineering; Signal transduction; Surfaces and Interfaces; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Thin Films; Growth Factor Stimulus; Cell Differentiation Assay; C2C12 Cell; Microarray Spot; Cell Differentiation Model; Cell culture; Prognosis; Cell differentiation; Biomedical engineering
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-012-4785-1

Cellular microarrays present a promising tool for multiplex evaluation of the signalling effect of substrate-immobilized factors on cellular differentiation. In this paper, we compare the early myoblast-to-osteoblast cell commitment steps in response to a growth factor stimulus using standard well plate differentiation assays or cellular microarrays. Our results show that restraints on the cell culture size, inherent to cellular microarrays, impair the differentiation outcome. Also, while cells growing on spots with immobilised BMP-2 are early biased towards the osteoblast fate, longer periods of cell culturing in the microarrays result in cell proliferation and blockage of osteoblast differentiation. The results presented here raise concerns about the efficiency of cell differentiation when the cell culture dimensions are reduced to a simplified microspot environment. Also, these results suggest that further efforts should be devoted to increasing the complexity of the microspots composition, aiming to replace signalling cues missing in this system.