Exploration of the relationship between the tumor burden and the concentration of vascular endothelial growth factor in liver-cancer-bearing animals using immunomagnetic reduction assay

To achieve early-stage diagnosis, a high-sensitivity assay method is needed. As a biomarker, vascular endothelial growth factor (VEGF) has played a growing role in diagnosing and treating hepatocellular carcinoma (HCC). In this work, an immunomagnetic reduction (IMR) through bio-functionalized magne...

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Published inJournal of biomedical nanotechnology Vol. 7; no. 4; p. 535
Main Authors Huang, K W, Yang, S Y, Yu, C Y, Chieh, J J, Yang, Che-Chuan, Horng, Herng-Er, Hong, Chin-Yih, Yang, Hong-Chang, Wu, Chau-Chung
Format Journal Article
LanguageEnglish
Published United States 01.08.2011
Subjects
Animals
Biomarkers, Tumor - blood
Blood Chemical Analysis - methods
Enzyme-Linked Immunosorbent Assay
Liver Neoplasms, Experimental - blood
Liver Neoplasms, Experimental - pathology
Magnetics - methods
Magnetite Nanoparticles
Male
Nanotechnology
Rats
Rats, Wistar
Tumor Burden
Vascular Endothelial Growth Factor A - blood
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Summary:To achieve early-stage diagnosis, a high-sensitivity assay method is needed. As a biomarker, vascular endothelial growth factor (VEGF) has played a growing role in diagnosing and treating hepatocellular carcinoma (HCC). In this work, an immunomagnetic reduction (IMR) through bio-functionalized magnetic nanoparticles and a high-temperature superconducting-quantum-interference-device magnetometer were utilized for quantitative detection of low-concentration VEGF in serum from rats with HCC. The precision and accuracy of IMR on VEGF were characterized. Further, the results of assaying VEGF in the serum of rats were compared with those of using enzyme-linked immunosorbant assay (ELISA). It was found the correlations between the detected VEGF concentration in the rat serum and tumor burdens were 0.99 and 0.90 for IMR and ELISA, respectively, within the range from 2 pg/ml to 8000 pg/ml of VEGF concentration.
ISSN:1550-7033
1550-7041
DOI:10.1166/jbn.2011.1321