LESION SIMULATING DISEASE 1 is required for acclimation to conditions that promote excess excitation energy

• Published in: Plant physiology (Bethesda) Vol. 136; no. 1; pp. 2818 - 2830
• Main Authors: Mateo, A, Muhlenbock, P, Rusterucci, C, Chang, C.C.C, Miszalski, Z, Karpinska, B, Parker, J.E, Mullineaux, P.M, Karpinski, S
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Biologists 01.09.2004; American Society of Plant Physiologists
• Subjects: acclimation; Acclimatization; Agronomy. Soil science and plant productions; Arabidopsis - cytology; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis - radiation effects; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Base Sequence; Biological and medical sciences; catalase; Catalase - genetics; Catalase - metabolism; Cell death; Cell Death - genetics; chlorophyll a-b-binding harvesting-organelle specific mutants; DNA, Plant - genetics; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Economic plant physiology; Environmental Stress and Adaptation; enzyme activity; excess excitation energy; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; gas exchange; Generalities. Disease free stocks; Genes, Plant; hydrogen peroxide; Hypersensitive response; Leaves; lesion simulating disease 1 gene; Lesions; lesions (plant); Light; Metabolism; molecular sequence data; Mutation; Net assimilation, photosynthesis, carbon metabolism. Photorespiration, respiration, fermentation (anoxia, hypoxia); nucleotide sequences; Nutrition. Photosynthesis. Respiration. Metabolism; Pathogens; Pathology, epidemiology, host-fungus relationships. Damages, economic importance; Peronospora - pathogenicity; Peronospora parasitica; Phenotype; Phenotypes; phenotypic variation; Photorespiration; Photosynthesis, respiration. Anabolism, catabolism; photosystem II; Photosystem II Protein Complex - metabolism; Phytopathology. Animal pests. Plant and forest protection; plant morphology; Plant physiology and development; Plants; run away cell death; Salicylic Acid - pharmacology; Stomata; stomatal conductance; Transcription Factors - genetics; Transcription Factors - metabolism; Salicylic acid; Enzyme; Arabidopsis; Stomatal conductance; Stomata; Photoperiod; Chlorophyll a; Photosystem; Peroxidases; Catalase; Cruciferae; Photorespiration; Dicotyledones; Angiospermae; Stomatal movement; Spermatophyta; Oxidoreductases; Gas exchange; Mutation
• ISSN: 0032-0889; 1532-2548; 1532-2548
• DOI: 10.1104/pp.104.043646

The lsd1 mutant of Arabidopsis fails to limit the boundaries of hypersensitive cell death response during avirulent pathogen infection and initiates unchecked lesions in long day photoperiod giving rise to the runaway cell death (rcd) phenotype. We link here the initiation and propagation of rcd to the activity of photosystem II, stomatal conductance and ultimately to photorespiratory H2O2. A cross of lsd1 with the chlorophyll a/b binding harvesting-organelle specific (designated cao) mutant, which has a reduced photosystem II antenna, led to reduced lesion formation in the lsd1 / cao double mutant. This lsd1 mutant also had reduced stomatal conductance and catalase activity in short-day permissive conditions and induced H2O2 accumulation followed by rcd when stomatal gas exchange was further impeded. All of these traits depended on the defense regulators EDS1 and PAD4. Furthermore, nonphotorespiratory conditions retarded propagation of lesions in lsd1. These data suggest that lsd1 failed to acclimate to light conditions that promote excess excitation energy (EEE) and that LSD1 function was required for optimal catalase activity. Through this regulation LSD1 can influence the effectiveness of photorespiration in dissipating EEE and consequently may be a key determinant of acclimatory processes. Salicylic acid, which induces stomatal closure, inhibits catalase activity and triggers the rcd phenotype in lsd1, also impaired acclimation of wild-type plants to conditions that promote EEE. We propose that the roles of LSD1 in light acclimation and in restricting pathogen-induced cell death are functionally linked.