auts2 Features and Expression Are Highly Conserved during Evolution Despite Different Evolutionary Fates Following Whole Genome Duplication

• Published in: Cells (Basel, Switzerland) Vol. 11; no. 17; p. 2694
• Main Authors: Merdrignac, Constance, Clément, Antoine Emile, Montfort, Jérôme, Murat, Florent, Bobe, Julien
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 30.08.2022; MDPI
• Subjects: Addictive behaviors; Animals; Autism; Autism Spectrum Disorder - genetics; autism spectrum disorders (ASDs); Behavior; brain; Embryos; Evolution; Gene Duplication; Gene expression; Genome - genetics; Genomes; Genomics; Gonads; Independent study; Life Sciences; Maximum likelihood method; medaka; Neurodevelopmental disorders; neurodevelopmental disorders (NDDs); Oryzias - genetics; Phylogenetics; Proteins; Salmon; Software; Species; teleost; Trout; Zebrafish; Zebrafish - genetics; Zebrafish Proteins - genetics; United States > US; autism spectrum disorders (ASDs); medaka; neurodevelopmental disorders (NDDs); evolution; teleost; zebrafish; brain
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells11172694

The gene plays major roles during brain development and is associated with various neuropathologies including autism. Data in non-mammalian species are scarce, and the aim of our study was to provide a comprehensive analysis of evolution in teleost fish, which are widely used for in vivo functional analysis and biomedical purposes. Comparative genomics in 78 species showed that and originate from the teleost-specific whole genome duplication (TGD). which is highly similar to human AUTS2, was almost systematically retained following TGD. In contrast, which encodes for a shorter protein similar to a short human AUTS2 isoform, was lost more frequently and independently during evolution. RNA-seq analysis in 10 species revealed a highly conserved profile with predominant expression of both genes in the embryo, brain, and gonads. Based on protein length, conserved domains, and expression profiles, we speculate that the long human isoform functions were retained by , while the short isoform functions were retained by and/or , depending on the lineage/species. showed a burst in expression during medaka brain formation, where it was expressed in areas of the brain associated with neurodevelopmental disorders. Together, our data suggest a strong conservation of functions in vertebrates despite different evolutionary scenarios in teleosts.