Antibacterial and Antioxidant Activities of Derriobtusone A Isolated from Lonchocarpus obtusus

• Published in: BioMed research international Vol. 2014; no. 2014; pp. 1 - 9
• Main Authors: Pessoa, Otília Deusdênia Loiola, Cavada, Benildo Sousa, Henriques, Mariana, Pereira, Maria Olivia, Teixeira, Edson Holanda, Vasconcelos, Mayron Alves, Bandeira, Paulo Nogueira, dos Santos, Hélcio Silva, Albuquerque, Maria Rose Jane Ribeiro, Arruda, Francisco Vassiliepe Sousa, de Alencar, Daniel Barroso, Saker-Sampaio, Silvana
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01.01.2014; Hindawi Publishing Corporation; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - chemistry; Antioxidants; Antioxidants - administration & dosage; Antioxidants - chemistry; Artemia; Bacteria; Bacterial infections; Bacteriology; Beans; Bioengineering; Biofilms; Biofilms - drug effects; Bioflavonoids; Biomedical research; E coli; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - growth & development; Escherichia coli - pathogenicity; Flavones; Flavonoids; Flavonoids - administration & dosage; Flavonoids - chemistry; Flavonoids - isolation & purification; Flavonoids - pharmacology; Free radicals; Health aspects; Humans; Legal medicine; Legumes; Lonchocarpus; Medicinal plants; Microbial Sensitivity Tests; Microorganisms; Mimosaceae; Oxidation-Reduction; Oxidative stress; Plant Extracts - administration & dosage; Plant Extracts - chemistry; Science & Technology; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - growth & development; Staphylococcus aureus - pathogenicity; Studies; Toxicity
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2014/248656

This study evaluated the effect of derriobtusone A, a flavonoid isolated from Lonchocarpus obtusus, on two important pathogenic bacteria, Staphylococcus aureus and Escherichia coli, as well as its antioxidant activity and toxicity. Planktonic growth assays were performed, and the inhibition of biofilm formation was evaluated. In addition, antioxidant activity was assessed by DPPH radical scavenging assay, ferrous ion chelating assay, ferric-reducing antioxidant power assay, and β-carotene bleaching assay. Toxicity was evaluated by the brine shrimp lethality test. Results showed that derriobtusone A completely inhibited the planktonic growth of S. aureus at 250 and 500 μg/mL; however, it did not have the same activity on E. coli. Derriobtusone A reduced the biomass and colony-forming unit (cfu) of S. aureus biofilm at concentrations of 250 and 500 μg/mL. In various concentrations, it reduced the biofilm biomass of E. coli, and, in all concentrations, it weakly reduced the cfu. Derriobtusone A showed highly efficient antioxidant ability in scavenging DPPH radical and inhibiting β-carotene oxidation. The compound showed no lethality to Artemia sp. nauplii. In conclusion, derriobtusone A may be an effective molecule against S. aureus and its biofilm, as well as a potential antioxidant compound with no toxicity.