Determination of Lead in Serum by Graphite Furnace Atomic Absorption Spectrometry Using Chromium Nitrate as a Matrix Modifier

• Published in: Eisei kagaku Vol. 37; no. 1; pp. 22 - 27
• Main Authors: IMAI, SHOJI, HAYASHI, YASUHISA, SAITO, KENGO, TANAKA, TOSHIYUKI
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 1991; 公益社団法人日本薬学会; Nippon Yakugakkai
• Subjects: Biological and medical sciences; Chemical and industrial products toxicology. Toxic occupational diseases; graphite furnace atomic absorption spectrometry; lead; matrix modifier; Medical sciences; Metals and various inorganic compounds; serum; Toxicology; Assay; Lead; Serum; Investigation method; Blood
• ISSN: 0013-273X
• DOI: 10.1248/jhs1956.37.22

A method for the determination of lead in serum by graphite furnace atomic absorption spectrometry with the standard type graphite tube using chromium nitrate as a matrix modifier is described. Serum is used without complicated sample pretreatment but with dilution by 1% Triton X-100 of 0.1 mol/l nitric acid solution containing 2000 mgCr/l. Detection limit (S/N=3) was 0.22 μg/l in serum. Reproducibility (RSD) of 4% at n=10 was observed. Recovery of lead in a certified reference material, bovine blood was 104%. The average sensitivity for five different serum samples was 0.00304 abs per μg/l (RSD : 4%). Lead level in 1.3 μg/l of serum was observed in twenty sera of normal subjects.