Activation of retinoic acid receptor-dependent transcription by organochlorine pesticides

• Published in: Toxicology and applied pharmacology Vol. 202; no. 1; pp. 38 - 49
• Main Authors: Lemaire, Géraldine, Balaguer, Patrick, Michel, Serge, Rahmani, Roger
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 2005; Elsevier
• Subjects: Animals; Benzoates - pharmacology; Biological and medical sciences; COS Cells; Cytochrome P-450 Enzyme System - biosynthesis; Cytochrome P450RAI1; Enzyme Induction; HeLa Cells; Humans; Hydrocarbons, Chlorinated - toxicity; Insecticides - toxicity; Life Sciences; Medical sciences; Naphthalenes - pharmacology; Organochlorine pesticides; Pesticides, fertilizers and other agrochemicals toxicology; Receptors, Retinoic Acid - physiology; Retinoic Acid 4-Hydroxylase; Retinoic acid receptor; Retinoids - metabolism; Retinoids - pharmacology; Toxicology; Transcriptional Activation - drug effects; RAR; RARE; DMSO; GADPH; Organochlorine pesticides; TTNPB; CRBPII; P450RAI1; EDTA; ER; RXR; DR; DMEM; Retinoic acid receptor; EC 50; Cytochrome P450RAI1; PCR; Cytochrome; Transcription; Organochlorine compounds; Pesticides; RAR retinoic acid receptor; Cytochrome P450RA11; CYTOCHROME P450RAI1; RETINOIC ACID RECEPTOR; ORGANOCHLORINE PESTICIDES
• ISSN: 0041-008X; 1096-0333
• DOI: 10.1016/j.taap.2004.06.004

Five organochlorine pesticides, namely, chlordane, dieldrin, aldrin, endrin, and endosulfan, activate human retinoic acid receptor (RAR)-mediated gene transcription via a retinoic acid response element (RARE). Transactivation studies were performed with stable RARα, β, or γ reporter cell lines in which the RAR DNA-binding domain (DBD) was replaced by that of estrogen receptor α (ERα)?. Five of the organochlorine pesticides tested activated RARβ and RARγ but not RARα; their half-maximal luciferase activity (EC 50) was determined. Furthermore, that activity was RAR-specific and organochlorine pesticides did not activate the retinoid X receptor (RXR) pathway. However, competitive binding experiments with [ 3H]-CD367, a pan-RAR agonist, showed that only chlordane could bind RARβ and RARγ, albeit with low affinity. In addition, organochlorine pesticides strongly induce cytochrome P450RAI1 (P450RAI1), a key factor of retinoic acid level regulation in many tissues and whose expression and activity are strongly induced by retinoic acid. This study shows that organochlorine pesticides can activate two RAR homologues, with low-binding affinity. Although the agonistic potential of organochlorine pesticides is lower than that of ( E)-4-[2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)-1-propenyl] benzoic acid (TTNPB), they are able to induce RAR-mediated gene transcription as P450RAI1 and may disrupt the retinoid signaling pathway. Because these chemicals are extremely persistent and tend to accumulate in biological tissues, these results support the hypothesis that the increase in teratogenicity observed in some developing countries could be due to prolonged exposure to organochlorine pesticides ubiquitously present in the environment.