The yeast Red1 protein localizes to the cores of meiotic chromosomes

• Published in: The Journal of cell biology Vol. 136; no. 5; pp. 957 - 967
• Main Authors: Smith, A.V. (Yale University, New Haven, CT.), Roeder, G.S
• Format: Journal Article
• Language: English
• Published: United States Rockefeller University Press 10.03.1997; The Rockefeller University Press
• Subjects: ADN; Antibodies; APAREAMIENTO CROMOSOMICO; APPARIEMENT CHROMOSOMIQUE; Cell division; Cell Nucleolus - chemistry; Cells; Chromatids; CHROMOSOME; Chromosomes; Chromosomes, Fungal - chemistry; CROMOSOMAS; DNA-Binding Proteins - analysis; Fungal Proteins - analysis; Fungal Proteins - genetics; Genes; MEIOSE; MEIOSIS; Mutation; Nuclear Proteins; Pachytene stage; Prophase; PROTEINAS AGLUTINANTES; PROTEINE DE LIAISON; Proteins; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - chemistry; Saccharomyces cerevisiae - physiology; Saccharomyces cerevisiae Proteins; Spores, Fungal; Sporulation; Synaptonemal Complex; Yeast; Yeasts
• ISSN: 0021-9525; 1540-8140
• DOI: 10.1083/jcb.136.5.957

Mutants in the meiosis-specific RED1 gene of S. cerevisiae fail to make any synaptonemal complex (SC) or any obvious precursors to the SC. Using antibodies that specifically recognize the Red1 protein, Red1 has been localized along meiotic pachytene chromosomes. Red1 also localizes to the unsynapsed axial elements present in a zip1 mutant, suggesting that Red1 is a component of the lateral elements of mature SCs. Anti-Red1 staining is confined to the cores of meiotic chromosomes and is not associated with the loops of chromatin that lie outside the SC. Analysis of the spo11 mutant demonstrates that Redl localization does not depend upon meiotic recombination. The localization of Red1 has been compared with two other meiosis specific components of chromosomes, Hop1 and Zip1: Zip1 serves as a marker for synapsed chromosomes. Double labeling of wild-type meiotic chromosomes with anti-Zip1 and anti-Red1 antibodies demonstrates that Red1 localizes to chromosomes both before and during pachytene. Double labeling with anti-Hop1 and anti-Red1 antibodies reveals that Hop1 protein localizes only in areas that also contain Red1, and studies of Hop1 localization in a real null mutant demonstrate that Hop1 localization depends on Red1 function. These observations are consistent with previous genetic studies suggesting that Red1 and Hop1 directly interact. There is little or no Hop1 protein on pachytene chromosomes or in synapsed chromosomal regions