Short term signaling responses in roots of young soybean seedlings exposed to cadmium stress

• Published in: Journal of plant physiology Vol. 170; no. 18; pp. 1585 - 1594
• Main Authors: Chmielowska-Bąk, Jagna, Lefèvre, Isabelle, Lutts, Stanley, Deckert, Joanna
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 15.12.2013
• Subjects: abscisic acid; Base Sequence; bioinformatics; Cadmium; Cadmium - toxicity; Ethylene; ethylene production; Ethylenes - metabolism; Exposure; Gene expression; Gene Expression Regulation, Plant - drug effects; Genes; Glycine max; Glycine max - drug effects; Glycine max - enzymology; Glycine max - genetics; Glycine max - metabolism; metabolism; mitogen-activated protein kinase; nitric oxide; Nucleotide Motifs - genetics; Plant Proteins - genetics; Plant Proteins - metabolism; plant response; Plant Roots - drug effects; Plant Roots - genetics; Plant Roots - metabolism; Plants (organisms); polyamines; Polyamines - metabolism; promoter regions; Promoter Regions, Genetic - genetics; proteins; roots; seedlings; Seedlings - drug effects; Seedlings - enzymology; Seedlings - genetics; Seedlings - metabolism; signal transduction; Signal Transduction - drug effects; Signal Transduction - genetics; Signaling; Soybeans; stress response; Stress, Physiological - drug effects; Stress, Physiological - genetics; Stresses; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; NO; Cadmium; Transcription factors; CDPK; NR; PLC; MAPKK; Ethylene; MAPK; Gene expression; CAM; SAMDC; PCD; ACS; Signaling; DAO; SNP; ROS; PCR; SAM; calcium-dependent protein kinase; calmodulin; mitogen-activated protein kinase; sodium nitroprusside; reactive oxygen species; diamine oxidase; programmed cell death; polymerase chain reaction; phospholipase C; nitric oxide; nitrate reductase; 1-aminocycpropane-1-carboxylic acid synthase; S-adenosylmethionine decarboxylase; mitogen-activated protein kinase kinase; S-adenosynomethionine
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2013.06.019

In the present study, the expression of fourteen genes involved in various signal transduction pathways was examined in young soybean (Glycine max) seedlings exposed to cadmium at two concentrations (10mgL−1 and 25mgL−1) for short time periods (3, 6 and 24h). The results show that cadmium causes induction of genes encoding proteins involved in ethylene and polyamines metabolism, nitric oxide generation, MAPK cascades and regulation of other genes’ expression. The bioinformatic analysis of promoter sequences of Cd-inducible genes revealed that their promoters possess several regulative motifs associated with the plant response to stress factors and abscisic acid and ethylene signaling. The involvement of ethylene in the response of soybean seedlings to cadmium stress was further confirmed by the real-time analysis of ethylene production during 24h of CdCl2 treatment. The role of the described signaling elements in transduction of the cadmium signal in young soybean seedlings is discussed.