The role of dynamin in HIV type 1 Env-mediated cell-cell fusion

HIV-1 envelope glycoproteins are the key viral proteins that mediate HIV-1 entry and cell-cell fusion. In contrast to HIV-1 entry, the mechanism of HIV-1 Env-mediated cell-cell fusion is relatively unclear. This study demonstrated that dynasore, a dynamin inhibitor, suppressed HIV-1 Env-mediated cel...

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Bibliographic Details
Published inAIDS research and human retroviruses Vol. 27; no. 9; p. 1013
Main Authors Lai, Weihong, Huang, Li, Ho, Phong, Montefiori, David, Chen, Chin-Ho
Format Journal Article
LanguageEnglish
Published United States 01.09.2011
Subjects
Cell Fusion
Dynamin II - antagonists & inhibitors
Dynamin II - metabolism
env Gene Products, Human Immunodeficiency Virus - metabolism
HIV-1 - pathogenicity
Host-Pathogen Interactions
Hydrazones - metabolism
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Summary:HIV-1 envelope glycoproteins are the key viral proteins that mediate HIV-1 entry and cell-cell fusion. In contrast to HIV-1 entry, the mechanism of HIV-1 Env-mediated cell-cell fusion is relatively unclear. This study demonstrated that dynasore, a dynamin inhibitor, suppressed HIV-1 Env-mediated cell-cell fusion. Dynasore sensitivity of HIV-1 Env-mediated cell-cell fusion varied depending on the viral strains. Results from testing a panel of gp41 cytoplasmic tail truncation mutants suggested that the gp41 cytoplasmic tail might play a role in dynasore sensitivity. HIV-1 Env-mediated cell-cell fusion could also be suppressed by a dynamin dominant-negative mutant DNM2(K44A). In summary, these results suggested that dynamin 2 might play a role in HIV-1 Env-mediated cell-cell fusion.
ISSN:1931-8405
DOI:10.1089/aid.2010.0259