Superoxide dismutase and ascorbate peroxidase improve the recovery of photosynthesis in sugarcane plants subjected to water deficit and low substrate temperature

• Published in: Plant physiology and biochemistry Vol. 73; pp. 326 - 336
• Main Authors: Sales, Cristina R.G., Ribeiro, Rafael V., Silveira, Joaquim A.G., Machado, Eduardo C., Martins, Marcio O., Lagôa, Ana Maria M.A.
• Format: Journal Article
• Language: English
• Published: Paris Elsevier Masson SAS 01.12.2013; Elsevier
• Subjects: abiotic stress; Adaptation, Physiological; Adaptation, Physiological - genetics; antioxidant activity; antioxidants; Antioxidants - metabolism; ascorbate peroxidase; Ascorbate Peroxidases; Ascorbate Peroxidases - genetics; Ascorbate Peroxidases - metabolism; Biological and medical sciences; C4 plants; Cold; Cold Temperature; cultivars; drought; drought tolerance; Droughts; enzymology; Fundamental and applied biological sciences. Psychology; genetics; Genotype; heat; metabolism; Oxidative stress; Phenotype; Photochemical activity; Photosynthesis; Photosynthesis - genetics; photosystem II; Photosystem II Protein Complex; Photosystem II Protein Complex - metabolism; physiological response; Plant physiology and development; Plant Transpiration; Saccharum; Saccharum - enzymology; Saccharum - genetics; Saccharum - metabolism; Saccharum spp; Stress, Physiological; Stress, Physiological - genetics; sugarcane; superoxide dismutase; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; temperature; Water; Water deficit; Oxidative stress; ETR; C; D; E; Water deficit; PSII; ΔF/FM; Saccharum spp; APX; dag; H2O2; ETR/PN; NPQ; Ψw; R; RDM; PPFD; TDM; SDM; CD; Cold; Photochemical activity; FV/FM; SOD; TBARS; gs; EXC; CAT; ROS; Photosynthesis; PET; PN; Monocotyledones; Enzyme; L-Ascorbate peroxidase; Superoxide dismutase; Recovery; Sugar plant; Substrate; Peroxidases; Saccharum officinarum; Plant physiology; Gramineae; Angiospermae; Spermatophyta; Oxidoreductases; Low temperature; Photochemistry; stomatal conductance; thiobarbituric acid-reactive substances; HO; maximum quantum yield of PSII; cold + drought treatment; days after germination; ETR/P(N); catalase; actual quantum yield of PSII; non-photochemical quenching; ratio between the apparent electron transport rate and leaf CO assimilation; leaf water potential; hydrogen peroxide; ΔF/F(M); leaf CO assimilation; photosynthetic photon flux density; photosystem II; superoxide dismutase; Ψ(w); photosynthetic electron transport; ascorbate-peroxidase; shoot dry matter; F(V)/F(M); transpiration; apparent electron transport rate; P(N); reactive oxygen species; g(s); reference treatment; root dry matter; relative excessive photosynthetic photon flux density; total dry matter; drought treatment; cold treatment
• ISSN: 0981-9428; 1873-2690; 1873-2690
• DOI: 10.1016/j.plaphy.2013.10.012

The physiological responses of C4 species to simultaneous water deficit and low substrate temperature are poorly understood, as well as the recovery capacity. This study investigated whether the effect of these abiotic stressors is cultivar-dependent. The differential responses of drought-resistant (IACSP94-2094) and drought-sensitive (IACSP97-7065) sugarcane cultivars were characterized to assess the relationship between photosynthesis and antioxidant protection by APX and SOD isoforms under stress conditions. Our results show that drought alone or combined with low root temperature led to excessive energetic pressure at the PSII level. Heat dissipation was increased in both genotypes, but the high antioxidant capacity due to higher SOD and APX activities was genotype-dependent and it operated better in the drought-resistant genotype. High SOD and APX activities were associated with a rapid recovery of photosynthesis in IACSP94-2094 plants after drought and low substrate temperature alone or simultaneously. •Plant growth was not affected in the drought tolerant genotype.•The drought sensitive genotype reduced growth when subject to drought or cold + drought.•The constitutive SOD activity in the tolerant genotype was higher than in the sensitive.•The effects of water deficit and cold + drought simultaneously are cultivar dependent.