Differences in the Cell Type-Specific Toxicity of Diamond Nanoparticles to Endothelial Cells Depending on the Exposure of the Cells to Nanoparticles

• Published in: International journal of nanomedicine Vol. 18; pp. 2821 - 2838
• Main Authors: Wierzbicki, Mateusz, Zawadzka, Katarzyna, Wójcik, Barbara, Jaworski, Sławomir, Strojny, Barbara, Ostrowska, Agnieszka, Małolepszy, Artur, Mazurkiewicz-Pawlicka, Marta, Sawosz, Ewa
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2023; Dove; Dove Medical Press
• Subjects: Blood proteins; Cell Line; diamond nanoparticles; endothelial cells; Endothelial Cells - metabolism; Endothelium; Fibroblast growth factors; Humans; Infrared spectroscopy; NADP; Nanoparticles; Nanoparticles - chemistry; Nanoparticles - toxicity; nanotoxicity; Original Research; oxidative toxicity; Pharmaceutical industry; Protein biosynthesis; Raman spectroscopy; Reactive Oxygen Species - metabolism; Scientific equipment and supplies industry; Vascular endothelial growth factor; Poland; United Kingdom; United States; Germany; endothelial cells; nanotoxicity; diamond nanoparticles; oxidative toxicity
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S411424

Diamond nanoparticles are considered to be one of the most cytocompatible carbon nanomaterials; however, their toxicity varies significantly depending on the analysed cell types. The aim was to investigate the specific sensitivity of endothelial cells to diamond nanoparticles dependent on exposure to nanoparticles. Diamond nanoparticles were characterized with Raman spectroscopy, Fourier-transform infrared spectroscopy (FTIR) and dynamic light scattering (DLS). Toxicity of diamond nanoparticles was assessed for endothelial cells (HUVEC), human mammary epithelial cells (HMEC) and HS-5 cell line. The effect of diamond nanoparticles on the level of ROS, NO, NADPH and protein synthesis of angiogenesis-related proteins of endothelial cells was evaluated. Our studies demonstrated severe cell type-specific toxicity of diamond nanoparticles to endothelial cells (HUVEC) depending on nanoparticle surface interaction with cells. Furthermore, we have assessed the effect on cytotoxicity of the bioconjugation of nanoparticles with a peptide containing the RGD motive and a serum protein corona. Our study suggests that the mechanical interaction of diamond nanoparticles with the endothelial cell membranes and the endocytosis of nanoparticles lead to the depletion of NADPH, resulting in an intensive synthesis of ROS and a decrease in the availability of NO. This leads to severe endothelial toxicity and a change in the protein profile, with changes in major angiogenesis-related proteins, including VEGF, bFGF, ANPT2/TIE-2, and MMP, and the production of stress-related proteins, such as IL-6 and IL-8. We confirmed the presence of a relationship between the toxicity of diamond nanoparticles and the level of cell exposure to nanoparticles and the nanoparticle surface. The results of the study give new insights into the conditioned toxicity of nanomaterials and their use in biomedical applications.