Identification of metabolites of Radix Paeoniae Alba extract in rat bile, plasma and urine by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry

• Published in: Journal of pharmaceutical analysis Vol. 4; no. 1; pp. 14 - 25
• Main Authors: Chen, Zheng-Wei, Tong, Ling, Li, Shu-Ming, Li, Dong-Xiang, Zhang, Ying, Zhou, Shui-Ping, Zhu, Yong-Hong, Sun, He
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.02.2014; Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China%Tasly R&D Institute, Tianjin Tasly Group Co., Ltd., Tianjin 300402, China; Xi'an Jiaotong University
• Subjects: Metabolite profiling; Monoterpene glycosides; Original; Radix Paeoniae Alba; UPLC–Q-TOF/MS; 串联质谱; 代谢产物; 四极杆; 大鼠; 提取物; 胆汁; 超高效液相色谱; 飞行时间质谱法; UPLC–Q-TOF/MS; Metabolite profiling; Monoterpene glycosides; Radix Paeoniae Alba; UPLC-Q-TOF/MS
• ISSN: 2095-1779; 2214-0883
• DOI: 10.1016/j.jpha.2013.06.004

Ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry （UPLC-Q-TOF/MS） was developed to identify the absorbed parent components and metabolites in rat bile, plasma and urine after oral administration of Radix Paeoniae Alba extract （RPAE）. A total of 65 compounds were detected in rat bile, plasma and urine samples, including 11 parent compounds and 54 metabolites. The results indicated that glucuronidation, hydroxylation and methylation were the major metabolic pathways of the components of RPAE. Furthermore, the results of this work demonstrated that UPLC-Q-TOF/MS combined with MetaboLynx？ software and mass defect filtering （MDF） could provide unique high throughput capabilities for drug metabolism study, with excellent MS mass accuracy and enhanced MSE data acquisition. With the MSE technique, both precursor and fragment mass spectra can be simultaneously acquired by alternating between high and low collision energy during a single chromatographic run.