Cloning by functional expression of platelet-activating factor receptor from guinea-pig lung

• Published in: Nature (London) Vol. 349; no. 6307; pp. 342 - 346
• Main Authors: Honda, Zen-ichiro, Nakamura, Motonao, Miki, Ichiro, Minami, Michiko, Watanabe, Tsuyoshi, Seyama, Yousuke, Okado, Haruo, Toh, Hiroyuki, Ito, Kohji, Miyamoto, Terumasa, Shimizu, Takao
• Format: Journal Article
• Language: English
• Published: London Nature Publishing 24.01.1991; Nature Publishing Group
• Subjects: Amino Acid Sequence; Anaphylaxis; Anatomy & physiology; Animals; Asthma; Base Sequence; Biochemistry; Biological and medical sciences; Calcium; Calcium channels; Cell Line; Cloning; Cloning, Molecular; Complementary DNA; Deoxyribonucleic acid; DNA; DNA - genetics; Electrophysiology; Endotoxins; Fundamental and applied biological sciences. Psychology; G protein-coupled receptors; Gene Expression; Genetics; GTP-Binding Proteins; Guinea Pigs; Inflammation; Lethality; Ligands; Lipids; lung; Lung - chemistry; Lungs; Medical research; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Muscle contraction; Muscles; Oocytes; Oocytes - physiology; Pathogenesis; Phospholipase; Phospholipase C; Phospholipids; Platelet Activating Factor - analogs & derivatives; Platelet Activating Factor - antagonists & inhibitors; Platelet Activating Factor - pharmacology; Platelet Membrane Glycoproteins; Platelet-activating factor; Platelet-activating factor receptors; Proteins; Receptors; Receptors, Cell Surface - chemistry; Receptors, Cell Surface - genetics; Receptors, Cell Surface - physiology; Receptors, G-Protein-Coupled; Sequence Homology, Nucleic Acid; Signal transduction; Smooth muscle; Transduction; Transfection; Xenopus laevis; New York; United States > US; Japan; Heterologous system; Lung; Rodentia; G Protein; Primary structure; Gene expression; Biological activity; Platelet activating factor; Vertebrata; Membrane receptor; Mammalia; Guinea pig; Molecular cloning

Platelet-activating factor (PAF), a unique phospholipid mediator, possesses potent proinflammatory, smooth-muscle contractile and hypotensive activities, and appears to be crucial in the pathogenesis of bronchial asthma and in the lethality of endotoxin and anaphylactic shock. Despite this, little i...