Cloning by functional expression of platelet-activating factor receptor from guinea-pig lung

• Published in: Nature (London) Vol. 349; no. 6307; pp. 342 - 346
• Main Authors: Honda, Zen-ichiro, Nakamura, Motonao, Miki, Ichiro, Minami, Michiko, Watanabe, Tsuyoshi, Seyama, Yousuke, Okado, Haruo, Toh, Hiroyuki, Ito, Kohji, Miyamoto, Terumasa, Shimizu, Takao
• Format: Journal Article
• Language: English
• Published: London Nature Publishing 24.01.1991; Nature Publishing Group
• Subjects: Amino Acid Sequence; Anaphylaxis; Anatomy & physiology; Animals; Asthma; Base Sequence; Biochemistry; Biological and medical sciences; Calcium; Calcium channels; Cell Line; Cloning; Cloning, Molecular; Complementary DNA; Deoxyribonucleic acid; DNA; DNA - genetics; Electrophysiology; Endotoxins; Fundamental and applied biological sciences. Psychology; G protein-coupled receptors; Gene Expression; Genetics; GTP-Binding Proteins; Guinea Pigs; Inflammation; Lethality; Ligands; Lipids; lung; Lung - chemistry; Lungs; Medical research; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Muscle contraction; Muscles; Oocytes; Oocytes - physiology; Pathogenesis; Phospholipase; Phospholipase C; Phospholipids; Platelet Activating Factor - analogs & derivatives; Platelet Activating Factor - antagonists & inhibitors; Platelet Activating Factor - pharmacology; Platelet Membrane Glycoproteins; Platelet-activating factor; Platelet-activating factor receptors; Proteins; Receptors; Receptors, Cell Surface - chemistry; Receptors, Cell Surface - genetics; Receptors, Cell Surface - physiology; Receptors, G-Protein-Coupled; Sequence Homology, Nucleic Acid; Signal transduction; Smooth muscle; Transduction; Transfection; Xenopus laevis; New York; United States > US; Japan; Heterologous system; Lung; Rodentia; G Protein; Primary structure; Gene expression; Biological activity; Platelet activating factor; Vertebrata; Membrane receptor; Mammalia; Guinea pig; Molecular cloning
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/349342a0

Platelet-activating factor (PAF), a unique phospholipid mediator, possesses potent proinflammatory, smooth-muscle contractile and hypotensive activities, and appears to be crucial in the pathogenesis of bronchial asthma and in the lethality of endotoxin and anaphylactic shock. Despite this, little is known of the molecular properties of the PAF receptor and related signal transduction systems. Although several lines of evidence suggest that activation of the PAF receptor stimulates phospholipase C and subsequent inositol trisphosphate formation through G protein(s), the PAF receptor and calcium channel are reported to show a close relation. As a first approach to cloning lipid autacoid receptors, we have isolated complementary DNA for the PAF receptors. Our strategy involved gene expression in Xenopus laevis oocytes and electrophysiological detection of PAF-induced responses. Sequence analysis indicates that the receptor belongs to the superfamily of G protein-coupled receptors.