Naturally induced secretions of the potato cyst nematode co-stimulate the proliferation of both tobacco leaf protoplasts and human peripheral blood mononuclear cells

• Published in: Molecular plant-microbe interactions Vol. 12; no. 10; pp. 872 - 881
• Main Authors: Goverse, A, Rouppe van der Voort, J, Rouppe van der Voort, C, Kavelaars, A, Smant, G, Schots, A, Bakker, J, Helder, J
• Format: Journal Article
• Language: English
• Published: St Paul, MN APS Press 01.10.1999; The American Phytopathological Society
• Subjects: Adenine - analogs & derivatives; Adenine - pharmacology; Animals; auxins; Benzyl Compounds; benzyladenine; bioassays; biochemical pathways; Biological and medical sciences; cell cycle; cell differentiation; Cell Division; cytokinins; EPS; Fundamental and applied biological sciences. Psychology; Generalities; Globodera rostochiensis; Host parasite relation; pathogenicity; Humans; Invertebrates; Kinetin; Laboratorium voor Nematologie; Laboratory of Nematology; leaves; Leukocytes, Mononuclear - cytology; Leukocytes, Mononuclear - drug effects; molecular weight; monocytes; naphthaleneacetic acid; Naphthaleneacetic Acids - pharmacology; Nemathelminthia. Plathelmintha; Nematoda - physiology; Nicotiana - cytology; Nicotiana - drug effects; Nicotiana - physiology; Nicotiana tabacum; pathogenicity; peptides; phytohemagglutinins; Phytopathology. Animal pests. Plant and forest protection; Plant Growth Regulators - pharmacology; Plant Leaves; Plants, Toxic; protoplasts; Protoplasts - drug effects; Protoplasts - physiology; Protozoa. Invertebrates; Purines; roots; secretions; Solanum tuberosum - parasitology; Cell proliferation; Adenine(N; Purine derivatives; Auxin; Stimulant plant; Stimulation; Nicotiana tabacum; Pest; Mononuclear cell; Dicotyledones; Indoleacetic acid; Protoplast; Angiospermae; Plant growth substance; Solanaceae; benzyl; Nematoda; Human; Secretion; Host specificity; Globodera rostochiensis; Host agent relation; Cytokinin; In vitro; Helmintha; Nemathelminthia; Spermatophyta; Mitogen; Invertebrata
• ISSN: 0894-0282; 1943-7706
• DOI: 10.1094/MPMI.1999.12.10.872

Naturally induced secretions from infective juveniles of the potato cyst nematode Globodera rostochiensis costimulate the proliferation of tobacco leaf protoplasts in the presence of the synthetic phytohormones alpha-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP). With the use of a protoplast-based bioassay, a low-molecular-weight peptide(s) (<3 kDa) was shown to be responsible for the observed effect. This mitogenic oligopeptide(s) is functionally dissimilar to auxin and cytokinin and, in addition, it does not change the sensitivity of the protoplasts toward these phytohormones. In combination with the mitogen phytohemagglutinin (PHA), cyst nematode secretions also co-stimulated mitogenesis in human peripheral blood mononuclear cells (PBMC). The stimulation of plant cells isolated from nontarget tissue--these nematodes normally invade the roots of potato plants--suggests the activation of a general signal transduction mechanism(s) by an oligopeptide(s) secreted by the nematode. Whether a similar oligopeptide-induced mechanism underlies human PBMC activation remains to be investigated. Reactivation of the cell cycle is a crucial event in feeding cell formation by cyst nematodes. The secretion of a mitogenic low-molecular-weight peptide(s) by infective juveniles of the potato cyst nematode could contribute to the redifferentiation of plant cells into such a feeding cell.