Quantitative NMR for bioanalysis and metabolomics

Over the last several decades, significant technical and experimental advances have made quantitative nuclear magnetic resonance (qNMR) a valuable analytical tool for quantitative measurements on a wide variety of samples. In particular, qNMR has emerged as an important method for metabolomics studi...

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Bibliographic Details
Published inAnalytical and bioanalytical chemistry Vol. 404; no. 4; pp. 1165 - 1179
Main Authors Barding, Gregory A., Salditos, Ryan, Larive, Cynthia K.
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer-Verlag 01.09.2012
Springer
Subjects
Amino Acids - analysis
Analysis
Analytical Chemistry
Animals
Biochemistry
Biological
Biological Products - chemistry
Body Fluids - chemistry
Body Fluids - metabolism
Botanical
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Food Science
Humans
Laboratory Medicine
Magnetic Resonance Spectroscopy - methods
Mathematical analysis
Metabolites
Metabolomics
Metabolomics - methods
Monitoring/Environmental Analysis
Nuclear magnetic resonance
Proteins - chemistry
Review
Samples
Statistical analysis
Statistical methods
Isotope enrichment
Metabolomics
Bioanalysis
Natural products
qNMR
Metabonomics
HR-MAS
Biofluids
Tissues
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Summary:Over the last several decades, significant technical and experimental advances have made quantitative nuclear magnetic resonance (qNMR) a valuable analytical tool for quantitative measurements on a wide variety of samples. In particular, qNMR has emerged as an important method for metabolomics studies where it is used for interrogation of large sets of biological samples and the resulting spectra are treated with multivariate statistical analysis methods. In this review, recent developments in instrumentation and pulse sequences will be discussed as well as the practical considerations necessary for acquisition of quantitative NMR experiments with an emphasis on their use for bioanalysis. Recent examples of the application of qNMR for metabolomics/metabonomics studies, the characterization of biologicals such as heparin, antibodies, and vaccines, and the analysis of botanical natural products will be presented and the future directions of qNMR discussed.
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ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-012-6188-z