Cross-reactive IgE antibody responses to tropomyosins from Ascaris lumbricoides and cockroach

• Published in: Journal of allergy and clinical immunology Vol. 121; no. 4; pp. 1040 - 1046.e1
• Main Authors: Santos, Ana Beatriz R., PhD, Rocha, Gutemberg M., MD, Oliver, Constance, PhD, Ferriani, Virgínia P.L., MD, Lima, Rodrigo C., MD, Palma, Mário S., PhD, Sales, Valéria S.F., MD, Aalberse, Rob C., PhD, Chapman, Martin D., PhD, Arruda, L. Karla, MD
• Format: Journal Article
• Language: English
• Published: New York, NY Mosby, Inc 01.04.2008; Elsevier; Elsevier Limited
• Subjects: Adolescent; Adult; Age; Allergies; allergy; Allergy and Immunology; Amino Acid Sequence; Animals; Ascaris lumbricoides; Ascaris lumbricoides - chemistry; Ascaris lumbricoides - immunology; Asthma; Asthma - immunology; Asthma - metabolism; Biological and medical sciences; Child; Child, Preschool; Children & youth; Cloning; Cross Reactions; Crustaceans; Deoxyribonucleic acid; DNA; Dust; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Hypersensitivity - immunology; Hypersensitivity - metabolism; IgE cross-reactivity; Immunoglobulin E - biosynthesis; Immunoglobulin E - metabolism; Immunopathology; Infections; Medical sciences; Middle Aged; Molecular Sequence Data; Mollusks; Parasites; Periplaneta - chemistry; Periplaneta - immunology; Periplaneta americana; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; Studies; Tropical diseases; Tropomyosin; Tropomyosin - chemistry; Tropomyosin - immunology; Tropomyosin - metabolism; Viral infections; Periplaneta americana; Ascaris lumbricoides; Tropomyosin; Geometric mean; GM; allergy; parasites; asthma; IgE cross-reactivity; Allergy; Insecta; IgE; Blattidae; Cockroach; Immunology; Cross reaction; Bronchus disease; Obstructive pulmonary disease; Nematoda; Dictyoptera; Humoral immunity; Lung disease; Immunopathology; Immune response; Respiratory disease; Antibody; Parasite; Parasitosis; Asthma; Infection; Arthropoda; Helmintha; Nemathelminthia; Invertebrata
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2007.12.1147

Background Evidence indicates that infection with Ascaris lumbricoides may promote development of allergy and asthma. Objective To study the role of tropomyosin, a pan-allergen in invertebrates, in IgE responses to A lumbricoides. Methods Recombinant A lumbricoides and Periplaneta americana tropomyosins were expressed in Pichia pastoris . Levels of IgE to tropomyosins from A lumbricoides and P americana were determined by chimeric ELISA in sera from 119 children living in a parasite-endemic area and 112 patients with cockroach allergy from the allergy clinics. Presence of tropomyosin in A lumbricoides larvae at L3 stage was evaluated by immunofluorescence using mAb 1A6, directed against mite tropomyosin. Molecular modeling of P americana and A lumbricoides tropomyosins was performed by using the MODELLER program. Results A lumbricoides tropomyosin showed 69% to 98% sequence identity to tropomyosins from other invertebrates. The predicted structure of A lumbricoides tropomyosin was similar to that of P americana tropomyosin and showed the characteristic coiled-coil structure. Strong correlation was found for IgE antibodies to tropomyosins from A lumbricoides and P americana in sera from children living in a parasite-endemic area and from patients with cockroach allergy. Larvae of A lumbricoides reacted strongly with mAb 1A6. Conclusion Tropomyosin induces IgE responses in A lumbricoides –infected children and in patients allergic to cockroach.