Pyroglutamylproline purified from fermented barley extract increases ABCG2 expression in Caco-2 cells

Fermented barley extract P (FBEP) prepared from barley shochu distillation by-product has been reported to lower serum uric acid (UA) by increasing urinary excretion of UA. ATP-binding cassette subfamily G member 2 (ABCG2) is a transporter responsible for UA efflux from the intestinal tract and kidn...

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Bibliographic Details
Published inFood Science and Technology Research Vol. 29; no. 4; pp. 309 - 318
Main Authors Uehara, Eriko, Hokazono, Hideki
Format Journal Article
LanguageEnglish
Published Tsukuba Japanese Society for Food Science and Technology 01.01.2023
Japan Science and Technology Agency
Subjects
ABCG2
Barley
Caco-2
Distillation
Efflux
Epithelium
Excretion
High performance liquid chromatography
Intestine
Liquid chromatography
pyroglutamylproline
Shochu
Uric acid
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Summary:Fermented barley extract P (FBEP) prepared from barley shochu distillation by-product has been reported to lower serum uric acid (UA) by increasing urinary excretion of UA. ATP-binding cassette subfamily G member 2 (ABCG2) is a transporter responsible for UA efflux from the intestinal tract and kidneys. This study aimed to identify, among the compounds isolated from FBEP using high-performance liquid chromatography, the components that promote ABCG2 expression in Caco-2 cells. Pyroglutamylproline (pEP) increased the expression of ABCG2 in Caco-2 cells at the gene and protein levels. These results suggest that the pEP contained in FBEP may decrease serum UA levels by increasing the expression of urate efflux transporters in the intestinal epithelium and promoting UA excretion.
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ISSN:1344-6606
1881-3984
DOI:10.3136/fstr.FSTR-D-22-00213