An actin-regulated importin α/β-dependent extended bipartite NLS directs nuclear import of MRTF-A

• Published in: The EMBO journal Vol. 29; no. 20; pp. 3448 - 3458
• Main Authors: Pawłowski, Rafał, Rajakylä, Eeva Kaisa, Vartiainen, Maria K, Treisman, Richard
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 20.10.2010; Nature Publishing Group UK; Nature Publishing Group
• Subjects: actin; Actins - metabolism; Active Transport, Cell Nucleus - physiology; alpha Karyopherins - genetics; alpha Karyopherins - metabolism; Amino Acid Sequence; Animals; beta Karyopherins - genetics; beta Karyopherins - metabolism; Cell Nucleus - metabolism; EMBO20; EMBO37; importin; Mice; Molecular Sequence Data; MRTF; NIH 3T3 Cells; NLS; Nuclear Localization Signals - genetics; Nuclear Localization Signals - metabolism; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; RNA Interference; SRF; Trans-Activators - genetics; Trans-Activators - metabolism; importin; actin; MRTF; NLS; SRF

Myocardin‐related transcription factors (MRTFs) are actin‐regulated transcriptional coactivators, which bind G‐actin through their N‐terminal RPEL domains. In response to signal‐induced actin polymerisation and concomitant G‐actin depletion, MRTFs accumulate in the nucleus and activate target gene t...