An actin-regulated importin α/β-dependent extended bipartite NLS directs nuclear import of MRTF-A

• Published in: The EMBO journal Vol. 29; no. 20; pp. 3448 - 3458
• Main Authors: Pawłowski, Rafał, Rajakylä, Eeva Kaisa, Vartiainen, Maria K, Treisman, Richard
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 20.10.2010; Nature Publishing Group UK; Nature Publishing Group
• Subjects: actin; Actins - metabolism; Active Transport, Cell Nucleus - physiology; alpha Karyopherins - genetics; alpha Karyopherins - metabolism; Amino Acid Sequence; Animals; beta Karyopherins - genetics; beta Karyopherins - metabolism; Cell Nucleus - metabolism; EMBO20; EMBO37; importin; Mice; Molecular Sequence Data; MRTF; NIH 3T3 Cells; NLS; Nuclear Localization Signals - genetics; Nuclear Localization Signals - metabolism; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; RNA Interference; SRF; Trans-Activators - genetics; Trans-Activators - metabolism; importin; actin; MRTF; NLS; SRF
• ISSN: 0261-4189; 1460-2075; 1460-2075
• DOI: 10.1038/emboj.2010.216

Myocardin‐related transcription factors (MRTFs) are actin‐regulated transcriptional coactivators, which bind G‐actin through their N‐terminal RPEL domains. In response to signal‐induced actin polymerisation and concomitant G‐actin depletion, MRTFs accumulate in the nucleus and activate target gene transcription through their partner protein SRF. Nuclear accumulation of MRTFs in response to signal is inhibited by increased G‐actin level. Here, we study the mechanism by which MRTF‐A enters the nucleus. We show that MRTF‐A contains an unusually long bipartite nuclear localisation signal (NLS), comprising two basic elements separated by 30 residues, embedded within the RPEL domain. Using siRNA‐mediated protein depletion in vivo , and nuclear import assays in vitro , we show that the MRTF‐A extended bipartite NLS uses the importin (Imp)α/β‐dependent import pathway, and that import is inhibited by G‐actin. Interaction of the NLS with the Impα–Impβ heterodimer requires both NLS basic elements, and is dependent on the Impα major and minor binding pockets. Binding of the Impα–Impβ heterodimer to the intact MRTF‐A RPEL domain occurs competitively with G‐actin. Thus, MRTF‐A contains an actin‐sensitive nuclear import signal. The transcriptional coactivator MRTF‐A/Mal binds G‐actin, which sequesters it in the cytoplasm. In this study, Treisman and colleagues identify an unusual bipartite nuclear localisation signal in MRTF‐A, and show that importin α/β‐mediated import through this NLS is directly inhibited by G‐actin binding.