Evaluation of stratus® CS Stat fluorimetric analyser for measurement of cardiac markers Troponin I (cTnI), creatine kinase MB (CK-MB), and myoglobin

• Published in: Journal of clinical laboratory analysis Vol. 15; no. 6; pp. 314 - 318
• Main Authors: Bénéteau-Burnat, Bénédicte, Baudin, Bruno, Vaubourdolle, Michel
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 2001
• Subjects: Chemistry, Clinical - methods; CK-MB; Creatine Kinase - blood; Creatine Kinase, MB Form; Fluorometry - instrumentation; Humans; immunoassay; Immunoassay - methods; Isoenzymes - blood; myoglobin; Myoglobin - blood; Original; Troponin I; Troponin I - blood
• ISSN: 0887-8013; 1098-2825
• DOI: 10.1002/jcla.1043

Myoglobin, CK‐MB, and Troponin I (cTnI) are cardiac muscle necrosis markers that are useful for detecting acute myocardial infarction (AMI). The Stratus® CS (Dade Behring, Inc.) is a discrete fluorimetric immunoassay analyser designed for the determination of the three cardiac markers from a single sample of whole blood or plasma. Overall analytical performances of the Stratus® CS provided by Dade Behring were evaluated according to the French Society of Clinical Biology guidelines. Within‐run imprecision (n = 20) for the three parameters at three levels gave values under 5%, whereas CVs for between‐run imprecision (n = 20) were under 6%. The sensitivities were 0.03 μg/L for cTnI and 0.4 μg/L for CK‐MB. Linearities extended from 0–50 μg/L for cTnI, 0–140 μg/L for CK‐MB, and 1–900 μg/L for myoglobin. The results, particularly those obtained on whole‐blood samples, correlated well with those obtained on Stratus® II. We did not find any interference with haemolysis, icterus, or lipemia. The system was very easy to use, and fulfills the requirements for the analysis of the three cardiac markers in patients with acute chest pain in emergency situations. J. Clin. Lab. Anal. 15:314–318, 2001. © 2001 Wiley‐Liss, Inc.