HPV genotypes detected in cervical cancers from Alaska Native women, 1980-2007

• Published in: International journal of circumpolar health Vol. 72; no. 1; pp. 21115 - 4
• Main Authors: Kelly, Janet J., Unger, Elizabeth R., Dunne, Eileen F., Murphy, Neil, Tiesinga, James, Koller, Kathy R., Swango-Wilson, Amy, Philemonof, Dino, Lounmala, Xay, Markowitz, Lauri E., Steinau, Martin, Hennessy, Thomas
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01.01.2013; Co-Action Publishing; Taylor & Francis Group
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Alaska - epidemiology; Alaska Native; cervical cancer; Female; Genotype; Genotyping Techniques; HPV; HPV genotypes; Human papillomavirus 16 - genetics; Human papillomavirus 18 - genetics; Humans; Indians, North American - statistics & numerical data; Middle Aged; Papillomaviridae - genetics; Papillomavirus Infections - epidemiology; Papillomavirus Infections - genetics; Supplement 1, 2013; Uterine Cervical Neoplasms - epidemiology; Uterine Cervical Neoplasms - virology; Young Adult; Alaska; HPV genotypes; Alaska Native; cervical cancer; HPV
• ISSN: 2242-3982; 1239-9736; 2242-3982
• DOI: 10.3402/ijch.v72i0.21115

Human papillomavirus (HPV) vaccine prevents cervical pre-cancers and cancers caused by HPV types 16 and 18. This study provides information on the HPV types detected in cervical cancers of Alaska Native (AN) women. Cases of invasive cervical cancer diagnosed in AN women aged 18 and above between 1980 and 2007 were identified from the Alaska Native Tumor Registry. A representative formalin-fixed, paraffin-embedded archived pathology block was retrieved and serially sectioned to allow histologic confirmation of lesion (first and last sections) and PCR testing of intervening sections. Extracted DNA was tested for HPV using Linear Array HPV Genotyping Test (Roche Diagnostics) with additional INNO-LiPA HPV Genotyping Assay (Innogenetics) testing on negative or inadequate specimens. All specimens were tested for a minimum 37 HPV types. Of 62 cervical cancer specimens evaluated, 57 (91.9%) contained one or more HPV types. Thirty-eight (61.2%) cancers contained HPV types 16 or 18, and 18 (29%) contained an oncogenic type other than type 16 or 18. Overall, almost two-thirds (61.2%) of the archived cervical cancers had detectible HPV types 16 or 18, a finding similar to studies of US women. As expected, a proportion of cancers would not be prevented by the current vaccines. HPV vaccination and cervical cancer screening are important prevention strategies for AN women.