Enhanced anti-tumor immunotherapy by silica-coated magnetic nanoparticles conjugated with ovalbumin

• Published in: International journal of nanomedicine Vol. 14; pp. 8235 - 8249
• Main Authors: Lee, Sung-Ju, Kim, Jong-Jin, Kang, Kyung-Yun, Paik, Man-Jeong, Lee, Gwang, Yee, Sung-Tae
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.10.2019; Dove; Dove Medical Press
• Subjects: Albumin; Analysis; Animals; antigen-delivery systems; Antigens; Antigens - administration & dosage; Cancer; CD4+ T cell; CD8+ T cell; Chickens; Cytokines; Cytokines - biosynthesis; dendritic cell; Dendritic cells; Dendritic Cells - drug effects; Dendritic Cells - immunology; Drug therapy; EG-7; Female; Immune response; Immunity; Immunotherapy; Lymphocytes; Lymphomas; Magnetite Nanoparticles - chemistry; Magnetite Nanoparticles - toxicity; Mice, Inbred C57BL; Nanoparticles; Neoplasms - immunology; Neoplasms - therapy; Original Research; ovalbumin; Ovalbumin - chemistry; silica-coated magnetic nanoparticles; Silicon dioxide; Silicon Dioxide - chemistry; T cells; Tumors; South Korea; CD4+ T cell; ovalbumin; EG-7; CD8+ T cell; silica-coated magnetic nanoparticles; dendritic cell; antigen-delivery systems
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S194352

The effective induction of an antigen-specific T cell immune response through dendritic cell activation is one of the key goals of tumor immunotherapy. In this study, efficient antigen-delivery carriers using silica-coated magnetic nanoparticles were designed and, their antigen-specific T cell immune response through dendritic cell activation investigated. The results showed that the silica-coated magnetic nanoparticles with conjugated ovalbumin enhanced the production of cytokines and antigen uptake in bone marrow-derived dendritic cells. Also, this induced an antigen-specific cytotoxic T lymphocyte (CTL) immune response and activated antigen-specific Th1 cell responses, including IL-2 and IFN-γ production and proliferation. We proved that the immune-stimulatory effects of silica-coated magnetic nanoparticles with conjugated ovalbumin were efficient in inhibiting of tumor growth in EG7-OVA (mouse lymphoma-expressing ovalbumin tumor-bearing mice model). Therefore, the silica-coated magnetic nanoparticles with conjugated ovalbumin are expected to be useful as efficient anti-cancer immunotherapy agents.