High-throughput method for the determination of nitroimidazoles in muscle samples by liquid chromatography coupled to mass spectrometry

• Published in: Analytical and bioanalytical chemistry Vol. 407; no. 15; pp. 4411 - 4421
• Main Authors: Rúbies, A., Sans, G., Kumar, P., Granados, M., Companyó, R., Centrich, F.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.06.2015; Springer; Springer Nature B.V
• Subjects: Acetonitrile; Analysis; Analytical Chemistry; Animals; Bans; Biochemistry; Characterization and Evaluation of Materials; Chemical properties; Chemistry; Chemistry and Materials Science; Chickens; Chromatography; Chromatography, High Pressure Liquid - methods; Eggs - analysis; Food; Food Analysis - methods; Food Science; Guidelines; High-Throughput Screening Assays - methods; Honey; Hormone and Veterinary Drug Residue Analysis; Kidneys; Laboratories; Laboratory Medicine; Limit of Detection; Liquid chromatography; Mass spectrometry; Mass Spectrometry - methods; Mathematical analysis; Meat - analysis; Monitoring/Environmental Analysis; Muscles; Muscles - chemistry; Nitroimidazoles; Nitroimidazoles - analysis; Origins; Poultry; Research Paper; Retina; Scientific imaging; Swine; Germany; Spain; Triple quadrupole; Liquid chromatography; Food samples; Mass spectrometry; Nitroimidazoles
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-014-8436-x

We present a high-throughput confirmatory method for the analysis of 11 nitroimidazoles in muscle samples (metronidazole, MNZ; dimetridazole, DMZ; ronidazole, RNZ; tinidazole, TNZ; carnidazole, CRZ; secnidazole semihydrate, SCZ; ornidazole, ORZ; metronidazole-hydroxy, MNZ-OH; ipronidazole, IPZ; ipronidazole-hydroxy, IPZ-OH; and dimetridazole-hydroxy, HMMNI). Extraction with acetonitrile is efficient and simple, with the majority of recoveries ranging between 65 and 101 %, and without the need for clean-up of the extracts. The chromatographic analysis is performed using UPLC-QqQ-MS in the MRM mode, with an electrospray source operated in positive mode. Total chromatographic analysis time is 12 min. This method has been fully validated in muscle according to Decision 657/2002/CE guidelines, and the CCα values obtained were in the range of 0.04–0.4 μg·kg −1 . The method is currently used for the analysis of muscle samples and has been tested in other matrices of animal origin, such as kidney, retina and egg, with excellent results.