Folic acid reverses uric acid crystal-induced surface OAT1 internalization by inhibiting RhoA activity in uric acid nephropathy

• Published in: Molecular medicine reports Vol. 13; no. 3; pp. 2385 - 2392
• Main Authors: WU, XINLIN, LIU, JIANXIANG, ZHANG, JIANQING, LIU, HENG, YAN, MIANSHENG, LIANG, BIRONG, XIE, HONGBO, ZHANG, SHIJUN, SUN, BAOGUO, ZHOU, HOUMING
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.03.2016; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: Animals; Care and treatment; Cell Membrane - metabolism; Cellular signal transduction; Creatinine; Crystallization; Crystals; Dietary supplements; Disease Models, Animal; Endocytosis - drug effects; Folic acid; Folic Acid - pharmacology; Guanosine; Health aspects; Internalization; Kidney diseases; Kidney Diseases - enzymology; Kidney Diseases - pathology; Kidneys; Kinases; Male; Nephropathy; OAT1; Organic Anion Transport Protein 1 - metabolism; Properties; Protein Transport; Rats, Sprague-Dawley; RhoA; rhoA GTP-Binding Protein - antagonists & inhibitors; rhoA GTP-Binding Protein - metabolism; RhoA protein; Rodents; Signal transduction; Triphosphatase; Urea; Uric acid; Uric Acid - pharmacology; uric acid crystals; United States > US; China
• ISSN: 1791-2997; 1791-3004
• DOI: 10.3892/mmr.2016.4837

To investigate how organic anion transporter (OAT)-1 is involved in uric acid nephropathy (UAN), a rat model for UAN was established and the serum uric acid, blood urea nitrogen and serum creatinine levels were all measured, and observed to be increased. It was additionally identified that in UAN rats the surface OAT1 expression levels were reduced. By treating HEK cells with monosodium urate (MSU) crystals, it was observed that the cells exhibited a reduction in OAT1 levels. Furthermore, MSU crystals were observed to recruit Ras homolog family member A (RhoA), a small guanosine triphosphatase, to the membrane and activate it. Following RhoA activation, the OAT1 internalization rate was identified to be increased. The dominant-negative RhoA N19 mutation was able to block MSU-induced OAT1 internalization, indicating that the process was RhoA-dependent. Finally, the results indicated that folic acid, a daily nutritional supplement, was capable of rescuing MSU-induced nephropathy and OAT1 internalization. These observations indicated that uric acid crystals were able to reduce the OAT1 membrane distribution through activating RhoA, and that folic acid was capable of preventing MSU-induced OAT1 relocation by inhibiting the RhoA signaling pathway.