Hyaluronic acid production and characterization by novel Bacillus subtilis harboring truncated Hyaluronan Synthase

• Published in: AMB Express Vol. 12; no. 1; p. 88
• Main Authors: Amjad Zanjani, Fatemeh Sadat, Afrasiabi, Shadi, Norouzian, Dariush, Ahmadian, Gholamreza, Hosseinzadeh, Sara Ali, Fayazi Barjin, Alireza, Cohan, Reza Ahangari, Keramati, Malihe
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 12.07.2022; Springer Nature B.V; SpringerOpen
• Subjects: Acid production; Bacillus subtilis; Biomedical and Life Sciences; Biopolymers; Biotechnology; Enzymes; Hyaluronan synthase; Hyaluronic acid; In vivo methods and tests; Industrial applications; Life Sciences; Microbial Genetics and Genomics; Microbiology; Molecular weight; Original; Original Article; Structure-function relationships; Transmembrane domain; Truncation; Viscoelasticity; Transmembrane domain; Hyaluronic acid; Hyaluronan synthase; Molecular weight; Truncation
• ISSN: 2191-0855; 2191-0855
• DOI: 10.1186/s13568-022-01429-3

Hyaluronic Acid (HA) is a natural biopolymer that has important physiological and industrial applications due to its viscoelastic and hydrophilic characteristics. The responsible enzyme for HA production is Hyaluronan synthase (HAS). Although in vitro structure–function of intact HAS enzyme has been partly identified, there is no data on in vivo function of truncated HAS forms. In the current study, novel recombinant Bacillus subtilis strains harboring full length (RBSFA) and truncated forms of SeHAS (RBSTr4 and RBSTr3) were developed and HA production was studied in terms of titer, production rate and molecular weight (Mw). The maximum HA titer for RBSFA, RBSTr4 and RBSTr3 was 602 ± 16.6, 503 ± 19.4 and 728 ± 22.9 mg/L, respectively. Also, the HA production rate was 20.02, 15.90 and 24.42 mg/L.h −1 , respectively. The findings revealed that RBSTr3 produced 121% and 137% more HA rather than RBSFA and RBSTr4, respectively. More interestingly, the HA Mw was about 60 kDa for all strains which is much smaller than those obtained in prior studies. Key points The strains containing truncated forms of SeHAS enzysme are able to produce HA. The HA from all recombinant strains was the same and low Mw. Deletion of C-terminal region of SeHAS was not effective on Mw.