A novel mechanism for the promotion of quercetin glycoside absorption by megalo α-1,6-glucosaccharide in the rat small intestine

• Published in: Food chemistry Vol. 136; no. 2; pp. 293 - 296
• Main Authors: Shinoki, Aki, Lang, Weeranuch, Thawornkuno, Charin, Kang, Hee-Kwon, Kumagai, Yuya, Okuyama, Masayuki, Mori, Haruhide, Kimura, Atsuo, Ishizuka, Satoshi, Hara, Hiroshi
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 15.01.2013; Elsevier
• Subjects: Absorption; Animals; bioavailability; Biological and medical sciences; Biological Availability; Food industries; Fundamental and applied biological sciences. Psychology; glycosides; Glycosides - metabolism; Humans; ingestion; Intestinal Absorption; Intestine, Small - metabolism; Male; Oligosaccharides - metabolism; polymerization; quercetin; Quercetin - metabolism; Quercetin glycoside; Rats; Rats, Wistar; small intestine; solubilization; α-1,6-Glucosaccharide; Rats; α-1,6-Glucosaccharide; Absorption; Quercetin glycoside; Rat; Rodentia; Flavonoid; Mechanism; Polyphenol; Vertebrata; Mammalia; Quercetin; Glycoside
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2012.08.028

► α-1,6-Glucosaccharides promote quercetin glycoside absorption in the small intestine. ► The saccharides enhance water-solubility of quercetin glycoside in the intestine. ► The effects of megalo-size saccharide are higher than those of oligo-size saccharide. The presence of an α-1,6-glucosaccharide enhances absorption of water-soluble quercetin glycosides, a mixture of quercetin-3-O-β-d-glucoside (Q3G, 31.8%), mono (23.3%), di (20.3%) and more d-glucose adducts with α-1,4-linkage to a d-glucose moiety of Q3G, in a ligated small intestinal loop of anesthetized rats. We prepared α-1,6-glucosaccharides with different degrees of polymerization (DP) enzymatically and separated them into a megalo-isomaltosaccharide-containing fraction (M-IM, average DP=11.0) and an oligo-isomaltosaccharide-containing fraction (O-IM, average DP=3.6). Luminal injection of either saccharide fraction promoted the absorption of total quercetin-derivatives from the small intestinal segment and this effect was greater for M-IM than O-IM addition. M-IM also increased Q3G, but not the quercetin aglycone, concentration in the water-phase of the luminal contents more strongly than O-IM. The enhancement of Q3G solubilization in the luminal contents may be responsible for the increases in the quercetin glucoside absorption promoted by α-1,6-glucosaccharides, especially that by M-IM. These results suggest that the ingestion of α-1,6-glucosaccharides promotes Q3G bioavailability.