An Arabidopsis thaliana lipoxygenase gene can be induced by pathogens, abscisic acid, and methyl jasmonate

• Published in: Plant physiology (Bethesda) Vol. 101; no. 2; pp. 441 - 450
• Main Authors: Melan, M.A, Dong, X, Endara, M.E, Davis, K.R, Ausubel, F.M, Peterman, T.K
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.02.1993
• Subjects: Abscisic Acid - pharmacology; Acetates - pharmacology; ACIDE ABSCISSIQUE; ACIDO ABSCISICO; Amino Acid Sequence; Amino acids; Arabidopsis - genetics; ARABIDOPSIS THALIANA; ARN MENSAJERO; ARN MESSAGER; Base Sequence; Biological and medical sciences; Blotting, Northern; Blotting, Southern; Cloning, Molecular; Complementary DNA; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; Cyclopentanes - pharmacology; DNA; DNA, Complementary - genetics; Enzyme Induction - drug effects; Enzymes; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genes, Plant - genetics; Genome; Genomics; GRAINE; INHIBIDORES DEL CRECIMIENTO; LIPOXIGENASA; LIPOXYGENASE; Lipoxygenase - genetics; Messenger RNA; Metabolism; Molecular Biology and Gene Regulation; Molecular Sequence Data; Oxylipins; Pathogens; Plant Diseases - genetics; Plant physiology and development; Plant roots; Plants; PODER PATOGENO; POUVOIR PATHOGENE; PROTEINAS; PROTEINE; Pseudomonas - pathogenicity; PSEUDOMONAS SYRINGAE; RETARDATEUR DE CROISSANCE; RNA; RNA - isolation & purification; SECUENCIA NUCLEICA; SEMILLA; Sequence Analysis, DNA; Sequence Homology, Amino Acid; SEQUENCE NUCLEIQUE; Soybeans; Tissue Distribution; Virulence; Pseudomonadales; Plant pathogen; Nucleotide sequence; Enzyme; Induction; Gene expression; Arabidopsis thaliana; Characterization; Regulation(control); Cruciferae; Pseudomonas syringae; Dicotyledones; DNA; Angiospermae; Abscisic acid; Bacteria; Pseudomonadaceae; Isolation; Spermatophyta; Experimental plant; Lipoxygenase
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.101.2.441

We isolated and characterized a 2.8-kb, full-length, Arabidopsis thaliana cDNA clone encoding a lipoxygenase. DNA sequence analysis showed that the deduced amino acid sequence of the Arabidopsis protein is 72 to 78% similar to that of legume seed lipoxygenases. DNA blot analysis indicated that Arabidopsis contains a single gene, LOX1, with appreciable homology to the cDNA clone. RNA blot analysis showed that the LOX1 gene is expressed in Arabidopsis leaves, roots, inflorescences, and young seedlings. LOX1 expression levels were highest in roots and young seedlings. in mature plants, LOX1 mRNA levels increased upon treatment with the stress-related hormones abscisic acid and methyl jasmonate and remained high for at least 96 h. Expression of the LOX1 gene was examined following infiltration of leaves with virulent (Psm ES4326) and avirulent (Pst MM1065) strains of Pseudomonas syringae. LOX1 mRNA levels were induced approximately 6-fold by both virulent and avirulent strains; however, the response to avirulent strains was much more rapid. Infiltration of leaves with Pst MM1065 resulted in maximal induction within 12 h, whereas maximal induction by Psm ES4326 did not occur until 48 h. When a cloned avr gene, avrRpt2, was transferred to Psm ES4326, LOX1 mRNA accumulated in a pattern similar to that observed for the avirulent strain Pst MM1065