Synthesis, Antifungal Activity, and Cytotoxicity of AgBr-NP@CTMAB Hybrid and Its Application in PMMA

• Published in: International journal of nanomedicine Vol. 16; pp. 3091 - 3103
• Main Authors: Zhang, Qiao-Jun, Liu, Yue, Zhang, Wen-Ting, Huang, Jing-Jing, Li, Hao-Hong, Lu, You-Guang, Zheng, Ming, Zheng, Da-Li
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2021; Dove; Dove Medical Press
• Subjects: agbr-np@ctmab; Antifungal agents; Antifungal Agents - chemical synthesis; Antifungal Agents - chemistry; Antifungal Agents - pharmacology; biofilm; Biofilms - drug effects; Biofilms - growth & development; c. albicans; Candida albicans - drug effects; Candida albicans - growth & development; Candida albicans - physiology; Cetrimonium - chemistry; Chemistry Techniques, Synthetic; cytotoxicity; Humans; Nanoparticles - chemistry; Nanotechnology; Original Research; polymethyl methacrylate; Polymethyl Methacrylate - chemistry; Polymethylmethacrylate; China; polymethyl methacrylate; C. albicans; AgBr-NP@CTMAB; cytotoxicity; biofilm
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S290673

To synthesize and determine the antifungal activity of AgBr-nanoparticles (NP) @CTMAB (cetyltrimethyl-ammonium bromide) against ( ) for use in the field of denture cleaning. The morphology and structure of AgBr-NP@CTMAB were characterized by IR, UV-Vis, XRD and SEM. The antifungal potential of AgBr-NP@CTMAB against was determined by colony formation assay and growth curve analysis. PMMA containing AgBr-NP@CTMAB was prepared, and the long-term antifungal efficacy was analyzed. The effect against biofilm was analyzed by SEM and OD600 , and the color changes of the specimens were observed by stereomicroscopy after 1 week of incubation. Cytotoxicity to human oral gingival fibroblasts and oral mucosal epithelial cells was detected by Cell Counting Kit-8 (CCK-8) in vitro. The compound showed a good crystalline phase, the presence of AgBr nanoparticles and the hybridization of CTMAB+ with AgBr-NPs. AgBr-NP@CTMAB showed significant antifungal activity against at concentrations of 10 μg/mL and 20 μg/mL. PMMA specimens containing AgBr-NP@CTMAB showed no long-term antifungal effect against biofilm. The clearance rate of attached to PMMA was 44.73% after soaking in 10 µg/mL AgBr-NP@CTMAB solution for 30 min and 91.35% for 8 h. There was no significant residual cytotoxicity or visual color change after soaking. AgBr-NP@CTMAB showed promising potential treatment for denture cleaners.