Low podoplanin expression of tumor cells predicts poor prognosis in pathological stage IB squamous cell carcinoma of the lung, tissue microarray analysis of 136 patients using 24 antibodies

• Published in: Lung cancer (Amsterdam, Netherlands) Vol. 63; no. 3; pp. 418 - 424
• Main Authors: Ito, Takeo, Ishii, Genichiro, Nagai, Kanji, Nagano, Tatsuya, Kojika, Masakazu, Murata, Yukinori, Atsumi, Naho, Nishiwaki, Yutaka, Miyazaki, Eishi, Kumamoto, Toshihide, Ochiai, Atsushi
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ireland Ltd 01.03.2009; Elsevier
• Subjects: Adult; Age Factors; Aged; Aged, 80 and over; Antibodies, Neoplasm; Biological and medical sciences; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - pathology; D2-40; Dermatology; DNA, Neoplasm - genetics; Female; Follow-Up Studies; Gene Expression Regulation, Neoplastic; Hematology, Oncology and Palliative Medicine; Humans; Immunohistochemistry; Lung - metabolism; Lung - pathology; Lung cancer; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; Male; Medical sciences; Membrane Glycoproteins - biosynthesis; Membrane Glycoproteins - genetics; Middle Aged; Neoplasm Staging; Oligonucleotide Array Sequence Analysis - methods; Pneumology; Podoplanin; Prognosis; Pulmonary/Respiratory; Retrospective Studies; Risk Factors; Squamous cell carcinoma; Stage IB; Tissue microarray; Tumors; Tumors of the respiratory system and mediastinum; Tumors of the skin and soft tissue. Premalignant lesions; Immunohistochemistry; Squamous cell carcinoma; Prognosis; Lung cancer; Tissue microarray; D2-40; Stage IB; Podoplanin; Skin disease; Basal cell carcinoma; Lung; Tissue; Cancerology; Bronchus disease; Tumor cell; Pneumology; Human; Lung disease; Antibody; Respiratory disease; Prediction; Malignant tumor; Gene expression; Anatomic pathology; Low; Cancer
• ISSN: 0169-5002; 1872-8332
• DOI: 10.1016/j.lungcan.2008.06.008

Abstract The aim of this study was to identify clinicopathological and biological prognostic markers for patients who had undergone complete resection of pathological stage IB squamous cell carcinoma (SqCC) of the lung. A total of 136 consecutive stage IB SqCC patients fulfilled eligibility criteria, and their clinicopathological factors were evaluated. Tissue microarrays were also constracted, and immunohistochemical staining with 24 antibodies was performed. Correlations between clinicopathological factors, antibody immunohistochemical reactions, the patients’ overall survival (OS) and relapse-free survival (RFS) were evaluated. The univariate analysis showed that 70-year-old and over elderly group had a shorter OS time and RFS time than the younger group ( p = 0.0086 and p = 0.0091, respectively). The univariate analysis for immunohistochemical staining showed that the podoplanin-negative group had a shorter OS time and RFS time than the podoplanin-positive group ( p = 0.0106 and p = 0.0308, respectively). Multivariate analysis revealed a significant correlation between both the 70-year-old and over elderly group and the podoplanin-negative group and poor outcome (OS, p = 0.007 and p = 0.008, respectively; RFS, p = 0.008 and p = 0.024, respectively). The results showed that patient age and a novel biological prognostic marker, podoplanin, are useful for predicting a poor outcome of patients after complete resection of stage IB SqCC of the lung.