Antiviral activity of Alpinia katsumadai extracts against rotaviruses

• Published in: Research in veterinary science Vol. 92; no. 2; pp. 320 - 323
• Main Authors: Kim, Ha-Hyun, Kwon, Hyung-Jun, Ryu, Young Bae, Chang, Jong Sun, Cho, Kyoung-Oh, Hosmillo, Myra D.T., Rho, Mun-Chual, Park, Su-Jin, Lee, Woo Song
• Format: Journal Article
• Language: English
• Published: England Elsevier India Pvt Ltd 01.04.2012; Elsevier Limited; Published by Elsevier Ltd
• Subjects: Adsorption; Alpinia; Alpinia katsumadai; Animals; Anti-rotavirus; Cattle; Cattle Diseases - drug therapy; Cattle Diseases - virology; Diarrhea; Ethanol; Hemagglutination inhibition; Hemagglutination Inhibition Tests; Macaca mulatta; Phytotherapy - methods; Plant Extracts - therapeutic use; Proteins; Rotavirus; Rotavirus - drug effects; Rotavirus Infections - drug therapy; Rotavirus Infections - veterinary; Seeds; Swine; Swine Diseases - drug therapy; Swine Diseases - virology; Veterinary medicine; Viral adsorption; Viruses; Alpinia katsumadai; Viral adsorption; Hemagglutination inhibition; Anti-rotavirus
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2010.11.012

In vitro anti-rotavirus activity of Alpinia katsumadai (AK) extracts were evaluated against bovine G8P[7] and porcine G5P[7] rotaviruses in two different assay strategies, a mixed treatment assay and a post treatment assay. In the mixed treatment assay, six AK extracts [AK-1 (EtOH extract), AK-3 (H2O layer), AK-5 (40% methanol fraction), and AK-9–11 (H2O extract, polysaccharide fraction, supernatant fraction)] exhibited inhibitory activities against G5P[7] rotavirus with the EC50 values ranging from 0.7±0.4 to 33.7±6.5μg/mL. Extracts AK-1, AK-3, and AK-5 inhibited rotavirus infection against G8P[7] rotavirus, the with EC50 values of 8.4±2.2μg/mL, 6.5±0.8μg/mL and 8.4±5.0μg/mL, respectively. By hemagglutination inhibition (HI) assay, six AK extracts completely inhibited viral adsorption onto human RBCs in both strains of rotaviruses at less than 11μg/mL. However, in the post treatment assay, there was no anti activity shown against both strains of rotaviruses. As a result, six AK extracts were attributed mainly to having a strong interaction with hemagglutinin protein on the outer surface of rotavirus, resulting to blockage of viral adsorption.