Replication of Human Sapovirus in Human-Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Cells

Sapoviruses, like noroviruses, are single-stranded positive-sense RNA viruses classified in the family Caliciviridae and are recognized as a causative pathogen of diarrhea in infants and the elderly. Like human norovirus, human sapovirus (HuSaV) has long been difficult to replicate in vitro. Recentl...

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Published inViruses Vol. 15; no. 9; p. 1929
Main Authors Matsumoto, Naomi, Kurokawa, Shiho, Tamiya, Shigeyuki, Nakamura, Yutaka, Sakon, Naomi, Okitsu, Shoko, Ushijima, Hiroshi, Yuki, Yoshikazu, Kiyono, Hiroshi, Sato, Shintaro
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.09.2023
MDPI
Subjects
Antigens
Antisera
Bile
Care and treatment
Cell lines
Diagnosis
Diarrhea
Epidermal growth factor
Epithelial cells
Epithelium
Genomes
Genotypes
Health aspects
histo-blood group antigens
human sapovirus
Immunization
in vitro replication
induced pluripotent stem cell
Infections
intestinal epithelial cells
Intestine
Penicillin
Plasmids
Pluripotency
Replication
RNA viruses
Stem cells
Viruses
Japan
United States > US
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Summary:Sapoviruses, like noroviruses, are single-stranded positive-sense RNA viruses classified in the family Caliciviridae and are recognized as a causative pathogen of diarrhea in infants and the elderly. Like human norovirus, human sapovirus (HuSaV) has long been difficult to replicate in vitro. Recently, it has been reported that HuSaV can be replicated in vitro by using intestinal epithelial cells (IECs) derived from human tissues and cell lines derived from testicular and duodenal cancers. In this study, we report that multiple genotypes of HuSaV can sufficiently infect and replicate in human-induced pluripotent stem cell-derived IECs. We also show that this HuSaV replication system can be used to investigate the conditions for inactivation of HuSaV by heat and alcohol, and the effects of virus neutralization of antisera obtained by immunization with vaccine antigens, under conditions closer to the living environment. The results of this study confirm that HuSaV can also infect and replicate in human normal IECs regardless of their origin and are expected to contribute to future virological studies.
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ISSN:1999-4915
1999-4915
DOI:10.3390/v15091929