HD-ZIP I Transcription Factor (PtHB13) Negatively Regulates Citrus Flowering through Binding to FLOWERING LOCUS C Promoter

For floral induction in adult citrus, low temperature is one of the most important environmental factors. FLOWERING LOCUS C (FLC) plays a very important role in low-temperature-induced Arabidopsis flowering by repressed FLC expression under exposure to prolonged low-temperature conditions. However,...

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Published inPlants (Basel) Vol. 9; no. 1; p. 114
Main Authors Ma, Yu-Jiao, Li, Pei-Ting, Sun, Lei-Ming, Zhou, Huan, Zeng, Ren-Fang, Ai, Xiao-Yan, Zhang, Jin-Zhi, Hu, Chun-Gen
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 16.01.2020
MDPI
Subjects
Abscisic acid
adults
Arabidopsis
Citrus
Citrus fruits
Cloning
Ectopic expression
Environmental factors
flc
Flowering
Flowers & plants
Fruits
Genes
Homeobox
Homology
Leucine
leucine zipper
Leucine zipper proteins
Loci
Low temperature
seasonal variation
Seasonal variations
temperature
transcription (genetics)
Transcription factors
Transgenic plants
Woody plants
Yeast
yeasts
flowering
FLC
low temperature
FT
citrus
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Summary:For floral induction in adult citrus, low temperature is one of the most important environmental factors. FLOWERING LOCUS C (FLC) plays a very important role in low-temperature-induced Arabidopsis flowering by repressed FLC expression under exposure to prolonged low-temperature conditions. However, little is known about the FLC regulation mechanism in perennial woody plants such as citrus. In this study, the functions of citrus FLC homolog (PtFLC) were investigated by ectopic expression in Arabidopsis. Transcription factor of homeodomain leucine zipper I (HD-ZIP I) as an upstream regulator of PtFLC was identified by yeast one-hybrid screen to regulate its transcription. The HD-ZIP I transcription factor was highly homologous to Arabidopsis ATHB13 and thus was named PtHB13. Ectopically expressed PtHB13 inhibited flowering in transgenic Arabidopsis. Furthermore, the expression of PtFLC and PtHB13 showed a seasonal change during the floral induction period and was also affected by low temperature. Thus, we propose that PtHB13 binds to PtFLC promoter to regulate its activity during the citrus floral induction process.
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ISSN:2223-7747
2223-7747
DOI:10.3390/plants9010114