Pyruvate enhances oral tolerance via GPR31

• Published in: International immunology Vol. 34; no. 7; pp. 343 - 352
• Main Authors: Liu, Qizhi, Umemoto, Eiji, Morita, Naoki, Kayama, Hisako, Baba, Yoshihiro, Kurosaki, Tomohiro, Okumura, Ryu, Takeda, Kiyoshi
• Format: Journal Article
• Language: English
• Published: UK Oxford University Press 04.07.2022
• Subjects: bacterial metabolites; oral tolerance; IL-10; Treg; mucosal immunity
• ISSN: 1460-2377; 1460-2377
• DOI: 10.1093/intimm/dxac010

Abstract CX3CR1high myeloid cells in the small intestine mediate the induction of oral tolerance by driving regulatory T (Treg) cells. Bacterial metabolites, e.g. pyruvate and lactate, induce a dendrite extension of CX3CR1high myeloid cells into the intestinal lumen via GPR31. However, it remains unclear whether the pyruvate–GPR31 axis is involved in the induction of oral tolerance. Here, we show that pyruvate enhances oral tolerance in a GPR31-dependent manner. In ovalbumin (OVA)-fed Gpr31-deficient mice, an OVA-induced delayed-type hypersensitivity response was substantially induced, demonstrating the defective induction of oral tolerance in Gpr31-deficient mice. The percentage of RORγt+ Treg cells in the small intestine was reduced in Gpr31-deficient mice. In pyruvate-treated wild-type mice, a low dose of OVA efficiently induced oral tolerance. IL-10 production from intestinal CX3CR1high myeloid cells was increased by OVA ingestion in wild-type mice, but not in Gpr31-deficient mice. CX3CR1high myeloid cell-specific IL-10-deficient mice showed a defective induction of oral tolerance to OVA and a decreased accumulation of OVA-specific Treg cells in the small intestine. These findings demonstrate that pyruvate enhances oral tolerance through a GPR31-dependent effect on intestinal CX3CR1high myeloid cells. A pyruvate-GPR31 axis mediates oral tolerance Graphical Abstract Graphical Abstract