Primary chronic osteomyelitis of the jaw—a microbial investigation using cultivation and DNA analysis: a pilot study
Objectives The aims of this study were to investigate the infectious etiology of primary chronic osteomyelitis (PCO) of the jaw using an extraoral sampling approach and molecular methods. Study design In this pilot study, bone biopsies from the mandibles of 5 patients with PCO were sampled with an e...
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Published in | Oral surgery, oral medicine, oral pathology, oral radiology and endodontics Vol. 107; no. 5; pp. 641 - 647 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
New York, NY
Mosby, Inc
01.05.2009
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Objectives The aims of this study were to investigate the infectious etiology of primary chronic osteomyelitis (PCO) of the jaw using an extraoral sampling approach and molecular methods. Study design In this pilot study, bone biopsies from the mandibles of 5 patients with PCO were sampled with an extraoral sterile approach. Cultivation and polymerase chain reaction (PCR) were performed. Results Two of the biopsies yielded growth of Propionebacterium acnes. One biopsy also demonstrated Staphylococcus capitis. The biopsies with bacterial growth were also positive for the same bacteria by PCR analysis. Conclusion No specific bacterial etiology was demonstrated. However, the results of bacterial cultivation and PCR analysis were in agreement. Based on these findings, the molecular procedure used in this study can be considered to be suitable for identification of bacterial specimens in mandibular biopsies. Further studies are needed with larger patient populations to confirm these findings. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1079-2104 1528-395X |
DOI: | 10.1016/j.tripleo.2009.01.020 |