Sinu virus, a novel and divergent orthomyxovirus related to members of the genus Thogotovirus isolated from mosquitoes in Colombia

• Published in: Virology (New York, N.Y.) Vol. 501; pp. 166 - 175
• Main Authors: Contreras-Gutiérrez, María Angélica, Nunes, Marcio R.T, Guzman, Hilda, Uribe, Sandra, Gallego Gómez, Juan Carlos, Suaza Vasco, Juan David, Cardoso, Jedson F, Popov, Vsevolod L, Widen, Steven G, Wood, Thomas G, Vasilakis, Nikos, Tesh, Robert B
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.01.2017
• Subjects: Amino Acid Sequence; Animals; Colombia; Culicidae - virology; Evolution, Molecular; Genome, Viral; Infectious Disease; Insect-specific viruses; Models, Molecular; Molecular Sequence Data; Orthomyxoviridae; Orthomyxoviridae - chemistry; Orthomyxoviridae - classification; Orthomyxoviridae - genetics; Orthomyxoviridae - isolation & purification; Phylogeny; Sinu virus; Thogotovirus - chemistry; Thogotovirus - classification; Thogotovirus - genetics; Thogotovirus - isolation & purification; Thogotovirus genus; Viral Proteins - chemistry; Viral Proteins - genetics; Viral Proteins - metabolism; Orthomyxoviridae; Thogotovirus genus; Sinu virus; Insect-specific viruses
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/j.virol.2016.11.014

Abstract The genome and structural organization of a novel insect-specific orthomyxovirus, designated Sinu virus, is described. Sinu virus (SINUV) was isolated in cultures of C6/36 cells from a pool of mosquitoes collected in northwestern Colombia. The virus has six negative-sense ssRNA segments. Genetic analysis of each segment demonstrated the presence of six distinct ORFs encoding the following genes: PB2 (Segment 1), PB1, (Segment 2), PA protein (Segment 3), envelope GP gene (Segment 4), the NP (Segment 5), and M-like gene (Segment 6). Phylogenetically, SINUV appears to be most closed related to viruses in the genus Thogotovirus.