An optional surgical technique for obtaining lamellar donor grafts: a pilot study

• Published in: BMC ophthalmology Vol. 22; no. 1; p. 137
• Main Authors: Liu, Xin, Liu, Chunyu, Lin, Hui, Shao, Yuting, Zhang, Li, Bi, Yanlong
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 25.03.2022; BioMed Central; BMC
• Subjects: Animals; Conventional manual dissection; Cornea; Cornea - surgery; Corneal Stroma - surgery; Dissection; Endothelium; Keratoplasty; Lamellar donor tissue; Methods; Microscopy, Electron, Scanning; Ophthalmology; Pilot Projects; Reproducibility; Reversed manual dissection; Scanning electron microscopy; Surface quality; Surgery; Surgical techniques; Swine; Thickness uniformity; Tomography, Optical Coherence; United States > US; Germany; Conventional manual dissection; Surface quality; Reversed manual dissection; Keratoplasty; Thickness uniformity; Lamellar donor tissue
• ISSN: 1471-2415; 1471-2415
• DOI: 10.1186/s12886-022-02371-5

To evaluate the surface quality and thickness uniformity of lamellar donor grafts using an optional surgical technique called reversed manual dissection (RMD) in porcine corneas. Twenty-four paired porcine corneas (48 eyes) were numbered 1 to 24 and divided into 6 groups. All left corneas were assigned to conventional manual dissection (CMD), and all right corneas were assigned to RMD. Each group contained 8 corneas. For Groups I, II, and III, 30, 50, and 70% of the entire corneal thickness was dissected using CMD. For groups IV, V, and VI, 70, 50, and 30% of the entire corneal thickness was dissected using RMD. The residual stromal thickness was examined by anterior segment optical coherence tomography (ASOCT) to assess the thickness uniformity and scanning electron microscopy (SEM) to assess the surface quality. The thickness uniformity of the lamellar grafts between each paired group was not significantly different (p > 0.05). The qualitative surface roughness grading (QiSR) evaluated by masked observers through SEM was significantly higher in the RMD groups (p < 0.001). The quantitative surface roughness grading (QnSR) acquired from the Mountains software was significantly lower in the RMD groups (p < 0.001). RMD is an optional surgical technique for obtaining porcine lamellar grafts. The thickness uniformity of RMD is comparable to that of CMD, and a smoother surface with fewer ridges and roughness is achieved compared to CMD.