Structures of novel acidic galactooligosaccharides synthesized by Bacillus circulans beta-galactosidase

• Published in: Bioscience, biotechnology, and biochemistry Vol. 62; no. 9; pp. 1791 - 1794
• Main Authors: Yanahira, S. (Snow Brand Milk Products Co. Ltd., Kawagoe, Saitama (Japan). Technical Research Inst.), Yabe, Y, Nakakoshi, M, Miura, S, Matsubara, N, Ishikawa, H
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.09.1998; Japan Society for Bioscience Biotechnology and Agrochemistry
• Subjects: acidic galactooligosaccharides; BACILLUS; Bacillus - enzymology; Bacillus circulans; BETA GALACTOSIDASA; BETA GALACTOSIDASE; beta-Galactosidase - metabolism; Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; Carbohydrate Sequence; Fundamental and applied biological sciences. Psychology; Galactose; glucuronic acid; Methods. Procedures. Technologies; Molecular Sequence Data; N-acetylneuraminic acid; OLIGOSACARIDOS; OLIGOSACCHARIDE; OLIGOSACCHARIDES; Oligosaccharides - biosynthesis; Oligosaccharides - chemistry; β-galactosidase; Enzymatic synthesis; Glucuronic acid; Sialic acid; Purification; Bacillus circulans; Enzyme; Oligosaccharide; Chemical structure; Bacillaceae; Transglycosylation; Lactose; Bacillales; Health food; Glycosidases; Production; Bacteria; Hydrolases; O-Glycosidases; β-Galactosidase; Structural analysis

The structures of acidic oligosaccharides synthesized by a transglycosylation reaction by Bacillus circulans beta-galactosidase, using lactose as the galactosyl donor, and N-acetylneuraminic acid (NeuAc) and glucuronic acid (GlcUA) as the acceptors were investigated. Acidic oligosaccharides thus syn...