Structures of novel acidic galactooligosaccharides synthesized by Bacillus circulans beta-galactosidase

• Published in: Bioscience, biotechnology, and biochemistry Vol. 62; no. 9; pp. 1791 - 1794
• Main Authors: Yanahira, S. (Snow Brand Milk Products Co. Ltd., Kawagoe, Saitama (Japan). Technical Research Inst.), Yabe, Y, Nakakoshi, M, Miura, S, Matsubara, N, Ishikawa, H
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.09.1998; Japan Society for Bioscience Biotechnology and Agrochemistry
• Subjects: acidic galactooligosaccharides; BACILLUS; Bacillus - enzymology; Bacillus circulans; BETA GALACTOSIDASA; BETA GALACTOSIDASE; beta-Galactosidase - metabolism; Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; Carbohydrate Sequence; Fundamental and applied biological sciences. Psychology; Galactose; glucuronic acid; Methods. Procedures. Technologies; Molecular Sequence Data; N-acetylneuraminic acid; OLIGOSACARIDOS; OLIGOSACCHARIDE; OLIGOSACCHARIDES; Oligosaccharides - biosynthesis; Oligosaccharides - chemistry; β-galactosidase; Enzymatic synthesis; Glucuronic acid; Sialic acid; Purification; Bacillus circulans; Enzyme; Oligosaccharide; Chemical structure; Bacillaceae; Transglycosylation; Lactose; Bacillales; Health food; Glycosidases; Production; Bacteria; Hydrolases; O-Glycosidases; β-Galactosidase; Structural analysis
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.62.1791

The structures of acidic oligosaccharides synthesized by a transglycosylation reaction by Bacillus circulans beta-galactosidase, using lactose as the galactosyl donor, and N-acetylneuraminic acid (NeuAc) and glucuronic acid (GlcUA) as the acceptors were investigated. Acidic oligosaccharides thus synthesized were purified by anion exchange chromatography and charcoal chromatography. The MS and NMR studies indicated that the acidic oligosaccharides from NeuAc were Galbeta-(1 to 8)-NeuAc, Galbeta-(1 to 9)-NeuAc and Galbeta-(1 to 3)-Galbeta-(1 to 8)-NeuAc, and those from GlcUA were Galbeta-(1 to 3) GlcUA and Galbeta-(1 to 4) Galbeta-(1 to 3) GlcUA. These are novel acidic galactooligosaccharides