A simple and reliable new microchip electrophoresis method for fast measurements of imidazole dipeptides in meat from different animal species

• Published in: Analytical and bioanalytical chemistry Vol. 410; no. 18; pp. 4359 - 4369
• Main Authors: Jozanović, Marija, Sakač, Nikola, Sak-Bosnar, Milan, Carrilho, Emanuel
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.07.2018; Springer; Springer Nature B.V
• Subjects: Analytical Chemistry; Animal species; Biochemistry; Biological effects; Biological properties; Biological samples; Carnosine; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Electric potential; Electrophoresis; Evaluation; Food processing; Food Science; Food testing; Fraud; High-performance liquid chromatography; Imidazole; Imidazoles; Injection; Laboratory Medicine; Liquid chromatography; Measurement; Meat; Meat quality; Methods; Monitoring/Environmental Analysis; Peptides; Portable equipment; Research Paper; Semiconductors; Sensors; Separation; Species; Voltage; C; Electrochemical detection; Microchip electrophoresis; Imidazole dipeptides; Meat samples; D detector; Microfluidics; C4D detector
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-018-1087-6

Microchip electrophoresis (ME) was applied for the separation of two physiologically important imidazole dipeptides—carnosine and anserine. The capacitively coupled contactless conductivity detector (C 4 D) was employed for quantification of both dipeptides after separation in a new home-built ME unit. The separation parameters were optimized as follows to enable quantitative, baseline separation of both dipeptides: injection time 16 s, injection voltage 900 V/cm, and separation voltage 377.1 V/cm. The C 4 D detector responded linearly to both imidazole dipeptides in the range 0–20 mg L −1 . The known addition methodology was applied to test the accuracy of the measurement of imidazole dipeptides in a complex sample. The recoveries for measurement of carnosine in the mixture ranged from 96.1 to 105.0%, whereas those for anserine amounted to 96.6 to 102.0%. This method was also applied to real biological samples. The results exhibited a satisfactory agreement with a standard HPLC method. The proposed ME method represents a cheap, fast, and simple alternative to the existing, more complicated and expensive HPLC methods. This method does not demand either the optical detectors nor tedious derivatization of sample, which are unavoidable in HPLC methods. The method was succesfuly applied for animal species determination in unknown meat samples using the carnosine/anserine ratio, and subsequently, it could be used in a food fraud prevention process. Graphical abstract Microchip electrophoresis portable device with a C 4 D detector for determination of imidazole dipeptides in model samples and real meat samples from different animal species.