Characterization and identification of charcoal of inedible Kerandang fish ( Channa pleurophthalmus Blkr) body parts and potential antiallergenic properties

• Published in: Veterinary World Vol. 13; no. 7; pp. 1480 - 1486
• Main Authors: Aryani, Aryani, Suprayitno, Eddy, Sasmito, Bambang Budi, Hardoko, Hardoko
• Format: Journal Article
• Language: English
• Published: India Veterinary World 01.07.2020
• Subjects: Acetic acid; anti-allergy; Body weight; Channa pleurophthalmus; Charcoal; Chloroform; Enzyme-linked immunosorbent assay; Ethanol; Ethyl acetate; Fins; hyaluronidase; Immunoglobulin E; kerandang fish; Liquid chromatography; Mass spectroscopy; Ovalbumin; Scales; Scalp; hyaluronidase; charcoal; Kerandang fish; anti-allergy
• ISSN: 0972-8988; 2231-0916
• DOI: 10.14202/vetworld.2020.1480-1486

The study about the antiallergenic properties of inedible fish body parts is still limited. Therefore, this study aimed to characterize the charcoal from the body parts of Kerandang fish ( Blkr) and identify its antiallergenic properties. This study used some non-edible body parts extracted from the Kerandang fish (i.e., the scalp, scales, and dorsal, pectoral, ventral, anal, and caudal fins) using a maceration method with different solvents (ethanol, ethyl acetate, and chloroform). The identification of active compounds in the extract was carried out using liquid chromatography-high-resolution mass spectrometry (LC-HRMS) analysis, while the antihyaluronidase activity was determined using the antihyaluronidase test. The highest charcoal antihyaluronidase activity-extract was applied to ovalbumin-induced mice for 7 days with various doses (10, 15, and 20 mg/kg). The specific immunoglobulin E (IgE) was measured using enzyme-linked immunosorbent assay on day 8. Our LC-HRMS analysis showed that the active compound of charcoal in the caudal fins of Kerandang fish was hexadecanamide. The highest inhibition (IC ) of hyaluronidase was found in the ethyl acetate extract of fish caudal fins at a concentration of 4 mg/mL. We found that 15 mg/kg body weight of charcoal of fish caudal fins suppressed IgE expression in male mice. Our findings indicate that the charcoal of non-edible body parts of Kerandang and one of its constituent, hexadecanamide, may have strong antiallergic effects.