CRMP-5 interacts with actin to regulate neurite outgrowth

• Published in: Molecular medicine reports Vol. 13; no. 2; pp. 1179 - 1185
• Main Authors: GONG, XIAOBING, TAN, MINGHUI, GAO, YUAN, CHEN, KEEN, GUO, GUOQING
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.02.2016; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: Actin; Actins - metabolism; Adaptor Proteins, Signal Transducing - metabolism; Animal behavior; Animals; Axon guidance; Axonogenesis; CRMP-5; Cytoskeleton; Cytoskeleton - metabolism; Elongation; Genetic aspects; Growth; growth cone; Growth cones; Growth Cones - metabolism; HEK293 Cells; hippocampal neuron; Hippocampus; Humans; Immunocytochemistry; Immunoprecipitation; Laboratory animals; Microscopy; Microtubules; Nerve Tissue Proteins - metabolism; Nervous system; neurite growth; Neurites - metabolism; Neurogenesis; Phosphoproteins; Plasmids; Polarity; Properties; Protein Binding; Proteins; Rats, Sprague-Dawley; Rodents; siRNA; Studies; Tubulin; United States > US; China
• ISSN: 1791-2997; 1791-3004
• DOI: 10.3892/mmr.2015.4662

CRMP family proteins (CRMPs) are abundantly expressed in the developing nervous system mediating growth cone guidance, neuronal polarity and axon elongation. CRMP-5 has been indicated to serve a critical role in neurite outgrowth. However, the detailed mechanisms of how CRMP-5 regulates neurite outgrowth remain unclear. In the current study, co-immunoprecipitation was used to identify the fact that CRMP-5 interacted with the actin and tubulin cytoskeleton networks in the growth cones of developing hippocampal neurons. CRMP-5 exhibited increased affinity towards actin when compared with microtubules. Immunocytochemistry was used to identify the fact that CRMP-5 colocalized with actin predominantly in the C-domain and T-zone in growth cones. In addition, genetic inhibition of CRMP-5 by siRNA suppressed the expression of actin, growth cone development and neurite outgrowth. Overexpression of CRMP-5 promoted the interaction with actin, growth cone development and hippocampal neurite outgrowth. Taken together, these data suggest that CRMP-5 is able to interact with the actin cytoskeleton network in the growth cone and affect growth cone development and neurite outgrowth via this interaction in developing hippocampal neurons.