Genetic analysis of 430 Chinese Cynodon dactylon accessions using sequence-related amplified polymorphism markers

• Published in: International journal of molecular sciences Vol. 15; no. 10; pp. 19134 - 19146
• Main Authors: Huang, Chunqiong, Liu, Guodao, Bai, Changjun, Wang, Wenqiang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21.10.2014; MDPI
• Subjects: Breeding - methods; China; Cluster Analysis; Cynodon - genetics; Cynodon dactylon; DNA, Plant - genetics; Genetic diversity; Genetic Markers - genetics; genetic variation; Genetic Variation - genetics; Genome, Plant - genetics; Genotype & phenotype; Phylogeny; Plant populations; Polymorphism; Polymorphism, Genetic - genetics; Sequence Analysis - methods; SRAP markers; China
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms151019134

Although Cynodon dactylon (C. dactylon) is widely distributed in China, information on its genetic diversity within the germplasm pool is limited. The objective of this study was to reveal the genetic variation and relationships of 430 C. dactylon accessions collected from 22 Chinese provinces using sequence-related amplified polymorphism (SRAP) markers. Fifteen primer pairs were used to amplify specific C. dactylon genomic sequences. A total of 481 SRAP fragments were generated, with fragment sizes ranging from 260-1800 base pairs (bp). Genetic similarity coefficients (GSC) among the 430 accessions averaged 0.72 and ranged from 0.53-0.96. Cluster analysis conducted by two methods, namely the unweighted pair-group method with arithmetic averages (UPGMA) and principle coordinate analysis (PCoA), separated the accessions into eight distinct groups. Our findings verify that Chinese C. dactylon germplasms have rich genetic diversity, which is an excellent basis for C. dactylon breeding for new cultivars.