Evaluation of library preparation methods for Illumina next generation sequencing of small amounts of DNA from foodborne parasites

Illumina library preparation methods for ultra-low input amounts were compared using genomic DNA from two foodborne parasites (Angiostrongylus cantonensis and Cyclospora cayetanensis) as examples. The Ovation Ultralow method resulted in libraries with the highest concentration and produced quality s...

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Published inJournal of microbiological methods Vol. 130; pp. 23 - 26
Main Authors Nascimento, Fernanda S., Wei-Pridgeon, Yuping, Arrowood, Michael J., Moss, Delynn, da Silva, Alexandre J., Talundzic, Eldin, Qvarnstrom, Yvonne
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.11.2016
Subjects
Angiostrongylus cantonensis
Angiostrongylus cantonensis - genetics
Animals
Base Sequence
Cyclospora - genetics
Cyclospora cayetanensis
DNA, Protozoan - analysis
Food Parasitology
Foodborne Diseases - parasitology
Gene Library
Genes, Protozoan
Genome, Protozoan
High-Throughput Nucleotide Sequencing - instrumentation
High-Throughput Nucleotide Sequencing - methods
Illumina
Library preparation
MiSeq
Models, Biological
Next generation sequencing
Parasites - genetics
Cyclospora cayetanensis
Library preparation
Next generation sequencing
Illumina
MiSeq
Angiostrongylus cantonensis
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Summary:Illumina library preparation methods for ultra-low input amounts were compared using genomic DNA from two foodborne parasites (Angiostrongylus cantonensis and Cyclospora cayetanensis) as examples. The Ovation Ultralow method resulted in libraries with the highest concentration and produced quality sequencing data, even when the input DNA was in the picogram range. •Illumina genomic libraries were made using one nanogram or less of DNA.•Sequencing reads from ultra-low input resulted in quality genome assemblies.•The Ovation Ultralow method produced the best libraries for Illumina sequencing.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Present address: Office of Applied Research and Safety Assessment, Center for Food Safety and Nutrition, Food and Drug Administration, Laurel, MD 20708.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2016.08.020