GC-MS Characterization of Antibacterial, Antioxidant, and Antitrypanosomal Activity of Syzygium aromaticum Essential Oil and Eugenol

• Published in: Evidence-based complementary and alternative medicine Vol. 2021; pp. 6663255 - 12
• Main Authors: Teles, Amanda Mara, Silva-Silva, João Victor, Fernandes, Juan Matheus Pereira, Abreu-Silva, Ana Lucia, Calabrese, Kátia da Silva, Mendes Filho, Nestor Everton, Mouchrek, Adenilde Nascimento, Almeida-Souza, Fernando
• Format: Journal Article
• Language: English
• Published: United States Hindawi 2021; Hindawi Limited
• Subjects: Amastigotes; Antibiotics; Antimicrobial agents; Antioxidants; Aromatherapy; Bacterial infections; Biological activity; Chagas disease; Cytotoxicity; Epimastigotes; Essential oils; Ethanol; Eugenol; Flavonoids; Flowers & plants; Food; Gas chromatography; Infections; Laboratories; Mass spectroscopy; Microorganisms; Minimum inhibitory concentration; Oils & fats; Parasites; Phenolic compounds; Public health; Quality control; Syzygium aromaticum; Trypanosomiasis; Water quality; United States > US
• ISSN: 1741-427X; 1741-4288
• DOI: 10.1155/2021/6663255

Syzygium aromaticum has a diversity of biological activities due to the chemical compounds found in its plant products such as total phenolic compounds and flavonoids. The present work describes the chemical analysis and antimicrobial, antioxidant, and antitrypanosomal activity of the essential oil of S. aromaticum. Eugenol (53.23%) as the major compound was verified by gas chromatography-mass spectrometry. S. aromaticum essential oil was more effective against S. aureus (MIC 50 μg/mL) than eugenol (MIC 250 μg/mL). Eugenol presented higher antioxidant activity than S. aromaticum essential oil, with an EC50 of 12.66 and 78.98 µg/mL, respectively. S. aromaticum essential oil and eugenol exhibited Trypanosoma cruzi inhibitory activity, with IC50 of 28.68 ± 1.073 and 31.97 ± 1.061 μg/mL against epimastigotes and IC50 of 64.51 ± 1.658 and 45.73 ± 1.252 μg/mL against intracellular amastigotes, respectively. Both compounds presented low cytotoxicity, with S. aromaticum essential oil displaying 15.5-fold greater selectivity for the parasite than the cells. Nitrite levels in T. cruzi-stimulated cells were reduced by essential oil (47.01%; p = 0.002) and eugenol (48.05%; p = 0.003) treatment. The trypanocidal activity of S. aromaticum essential oil showed that it is reasonable to use it in future research in the search for new therapeutic alternatives for trypanosomiasis.