Protective effects of tanshinone IIA on endothelial progenitor cells injured by tumor necrosis factor-α

• Published in: Molecular medicine reports Vol. 12; no. 3; pp. 4055 - 4062
• Main Authors: WANG, XING-XIANG, YANG, JIN-XIU, PAN, YAN-YUN, ZHANG, YE-FEI
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.09.2015; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: Animals; Arteriosclerosis; Atherosclerosis; Care and treatment; CD40 antigen; CD40 Ligand - metabolism; Cell Adhesion - drug effects; Cell Movement - drug effects; Cell Proliferation - drug effects; Cells, Cultured; Chemokine CCL2 - metabolism; Chinese medicine; Development and progression; Diterpenes, Abietane - pharmacology; endothelial progenitor cell; Endothelial Progenitor Cells - cytology; Endothelial Progenitor Cells - drug effects; Endothelial Progenitor Cells - metabolism; Epidermal growth factor; Health aspects; Inflammation; Interleukin 6; Interleukin-6 - metabolism; Leukocyte migration; Male; Medicine, Chinese; Medicine, Chinese Traditional; Microscopy, Fluorescence; migration; Monocyte chemoattractant protein; Monocyte chemoattractant protein 1; Necrosis; paracrine; Paracrine signalling; Protective Agents - pharmacology; Rats; Rats, Sprague-Dawley; Rodents; Stem cells; Studies; tanshinone IIA; Traditional Chinese medicine; Tumor necrosis factor; Tumor Necrosis Factor-alpha - toxicity; Tumor necrosis factor-TNF; Tumor necrosis factor-α; vasculogenesis; China; United States > US
• ISSN: 1791-2997; 1791-3004
• DOI: 10.3892/mmr.2015.3969

Tanshinone IIA (Tan IIA) is a Traditional Chinese Medicine commonly used in Asian and Western countries for the prevention and treatment of cardiovascular disorders, such as atherosclerosis. Endothelial dysfunction and associated inflammatory processes have a critical role in the development of atherosclerosis. Endothelial progenitor cells (EPCs) have been demonstrated to be involved in certain aspects of the endothelial repair process. The present study aimed to investigate the putative protective effects of Tan IIA on EPCs injured by tumor necrosis factor-α (TNF-α). The potential effects of Tan IIA on TNF-α-stimulated EPC proliferation, migration, adhesion, in vitro tube formation ability and paracrine activity were investigated in the current study. The results indicated that TNF-α impaired EPC proliferation, migration, adhesion capacity and vasculogenesis ability in vitro as well as promoted EPC secretion of inflammatory cytokines, including monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6) and soluble CD40 ligand (sCD40L). However, Tan IIA was able to reverse these effects. In conclusion, these findings demonstrated that Tan IIA may have the potential to protect EPCs against damage induced by TNF-α. Therefore, these results may provide evidence for the pharmacological basis of Tan IIA and its potential use in the prevention and treatment of early atherosclerosis associated with EPC and endothelial damage.