Hsa_circ_0050102 regulates the pancreatic cancer development via miR‐218‐5p/PPME1 axis

• Published in: Journal of clinical laboratory analysis Vol. 36; no. 3; pp. e24247 - n/a
• Main Authors: Feng, Ningning, Jiao, Zhikai, Zhang, Yueshan, Yang, Baoming
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.03.2022; John Wiley and Sons Inc
• Subjects: Angiogenesis; Animals; Apoptosis; Binding sites; Cell adhesion & migration; Cell growth; Cell migration; Cell Proliferation - genetics; hsa_circ_0050102 migration; Humans; Laboratory animals; Malignancy; Metastasis; Mice; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; miR‐218‐5p; Pancreatic cancer; Pancreatic Neoplasms - genetics; Pheochromocytoma cells; Phosphoric Monoester Hydrolases; PPME1; Protein phosphatase; RNA, Circular - genetics; Teats; hsa_circ_0050102 migration; miR-218-5p; pancreatic cancer; PPME1
• ISSN: 0887-8013; 1098-2825
• DOI: 10.1002/jcla.24247

Background Pancreatic cancer (PC) is a malignancy worldwide. Circular RNAs (circRNAs) affects the growth of PC, nonetheless the mechanism is blurry. Here, we reconnoitered the parts of hsa_circ_0050102 in PC. Methods Hsa_circ_0050102, microRNA‐218‐5p (miR‐218‐5p) and protein phosphatase methylesterase 1 (PPME1) abundances were indicated by quantitative RT‐PCR or Western blot. Moreover, the cell functions were uncovered. Additionally, the relation of miR‐218‐5p and hsa_circ_0050102 or PPME1 was identified by dual‐luciferase reporter assay. Ultimately, the mice teats were utilized to quantity the part of hsa_circ_0050102. Results Hsa_circ_0050102 and PPME1 contents were increased, and the miR‐218‐5p was dwindled in PC. Hsa_circ_0050102 lack subdued cell vitality, colony formation, cell migration and invasion, and angiogenesis, but endorsed cell apoptosis in PC cells. Furthermore, miR‐218‐5p was established to block the development of PC cells via PPME1. Hsa_circ_0050102 bound to miR‐218‐5p to adjust the content of PPME1. Conclusion Hsa_circ_0050102 expedited the expansion of PC through growing PPME1 abundance by adjusting miR‐218‐5p. Circ_0050102 promoted proliferation, migration and invasion, and angiogenesis, while repressed cell apoptosis of PC cells via miR‐218‐5p/PPME1 axis.