Regulated Incorporation of Two Different Metal Ions into Programmed Sites in a Duplex by DNA Polymerase Catalyzed Primer Extension

Metal‐mediated base pairs formed by the coordination of metal ions to natural or artificial bases impart unique chemical and physical properties to nucleic acids and have attracted considerable interest in the field of nanodevices. AgI ions were found to mediate DNA polymerase catalyzed primer exten...

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Published in	Angewandte Chemie International Edition Vol. 53; no. 26; pp. 6624 - 6627
Main Authors	Funai, Tatsuya, Nakamura, Junko, Miyazaki, Yuki, Kiriu, Risa, Nakagawa, Osamu, Wada, Shun-ichi, Ono, Akira, Urata, Hidehito
Format	Journal Article
Language	English
Published	Weinheim WILEY-VCH Verlag 23.06.2014 WILEY‐VCH Verlag Wiley Subscription Services, Inc
Edition	International ed. in English
Subjects	Biocatalysis Deoxyribonucleic acid DNA DNA - chemistry DNA - metabolism DNA polymerase DNA structures DNA-Directed DNA Polymerase - chemistry DNA-Directed DNA Polymerase - metabolism Formations Genes Incorporation Ions - chemistry mercury Metal ions metal-mediated base pairing Metals - chemistry Metals - metabolism Nucleic Acid Conformation Nucleic acids Nucleotides Physical properties Recognition Silver silver DNA polymerase DNA structures mercury metal-mediated base pairing
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Abstract	Metal‐mediated base pairs formed by the coordination of metal ions to natural or artificial bases impart unique chemical and physical properties to nucleic acids and have attracted considerable interest in the field of nanodevices. AgI ions were found to mediate DNA polymerase catalyzed primer extension through the formation of a C–AgI–T base pair, as well as the previously reported C–AgI–A base pair. The comparative susceptibility of dNTPs to AgI‐mediated enzymatic incorporation into the site opposite cytosine in the template was shown to be dATP>dTTP≫dCTP. Furthermore, two kinds of metal ions, AgI and HgII, selectively mediate the incorporation of thymidine 5′‐triphosphate into sites opposite cytosine and thymine in the template, respectively. In other words, the regulated incorporation of different metal ions into programmed sites in the duplex by DNA polymerase was successfully achieved. (Quick) silver DNA: HgII and AgI ions were found to specifically trigger DNA polymerase catalyzed primer extension in the absence of a Watson–Crick matched nucleotide through the formation of T–HgII–T and newly discovered C–AgI–T base pairs. The strict base recognition by the metal ions enabled regulated incorporation of the two different metal ions into programmed sites in duplex DNA.
AbstractList	Metal-mediated base pairs formed by the coordination of metal ions to natural or artificial bases impart unique chemical and physical properties to nucleic acids and have attracted considerable interest in the field of nanodevices. Ag(I) ions were found to mediate DNA polymerase catalyzed primer extension through the formation of a C-Ag(I)-T base pair, as well as the previously reported C-Ag(I)-A base pair. The comparative susceptibility of dNTPs to Ag(I)-mediated enzymatic incorporation into the site opposite cytosine in the template was shown to be dATP>dTTP≫dCTP. Furthermore, two kinds of metal ions, Ag(I) and Hg(II), selectively mediate the incorporation of thymidine 5'-triphosphate into sites opposite cytosine and thymine in the template, respectively. In other words, the regulated incorporation of different metal ions into programmed sites in the duplex by DNA polymerase was successfully achieved. Metal‐mediated base pairs formed by the coordination of metal ions to natural or artificial bases impart unique chemical and physical properties to nucleic acids and have attracted considerable interest in the field of nanodevices. AgI ions were found to mediate DNA polymerase catalyzed primer extension through the formation of a C–AgI–T base pair, as well as the previously reported C–AgI–A base pair. The comparative susceptibility of dNTPs to AgI‐mediated enzymatic incorporation into the site opposite cytosine in the template was shown to be dATP>dTTP≫dCTP. Furthermore, two kinds of metal ions, AgI and HgII, selectively mediate the incorporation of thymidine 5′‐triphosphate into sites opposite cytosine and thymine in the template, respectively. In other words, the regulated incorporation of different metal ions into programmed sites in the duplex by DNA polymerase was successfully achieved. (Quick) silver DNA: HgII and AgI ions were found to specifically trigger DNA polymerase catalyzed primer extension in the absence of a Watson–Crick matched nucleotide through the formation of T–HgII–T and newly discovered C–AgI–T base pairs. The strict base recognition by the metal ions enabled regulated incorporation of the two different metal ions into programmed sites in duplex DNA. Metal-mediated base pairs formed by the coordination of metal ions to natural or artificial bases impart unique chemical and physical properties to nucleic acids and have attracted considerable interest in the field of nanodevices. Ag(I) ions were found to mediate DNA polymerase catalyzed primer extension through the formation of a C-Ag(I)-T base pair, as well as the previously reported C-Ag(I)-A base pair. The comparative susceptibility of dNTPs to Ag(I)-mediated enzymatic incorporation into the site opposite cytosine in the template was shown to be dATP>dTTP≫dCTP. Furthermore, two kinds of metal ions, Ag(I) and Hg(II), selectively mediate the incorporation of thymidine 5'-triphosphate into sites opposite cytosine and thymine in the template, respectively. In other words, the regulated incorporation of different metal ions into programmed sites in the duplex by DNA polymerase was successfully achieved. Abstract Metal‐mediated base pairs formed by the coordination of metal ions to natural or artificial bases impart unique chemical and physical properties to nucleic acids and have attracted considerable interest in the field of nanodevices. Ag I ions were found to mediate DNA polymerase catalyzed primer extension through the formation of a C–Ag I –T base pair, as well as the previously reported C–Ag I –A base pair. The comparative susceptibility of dNTPs to Ag I ‐mediated enzymatic incorporation into the site opposite cytosine in the template was shown to be dATP>dTTP≫dCTP. Furthermore, two kinds of metal ions, Ag I and Hg II , selectively mediate the incorporation of thymidine 5′‐triphosphate into sites opposite cytosine and thymine in the template, respectively. In other words, the regulated incorporation of different metal ions into programmed sites in the duplex by DNA polymerase was successfully achieved. Metal-mediated base pairs formed by the coordination of metal ions to natural or artificial bases impart unique chemical and physical properties to nucleic acids and have attracted considerable interest in the field of nanodevices. Ag super(I) ions were found to mediate DNA polymerase catalyzed primer extension through the formation of a C-Ag super(I)-T base pair, as well as the previously reported C-Ag super(I)-A base pair. The comparative susceptibility of dNTPs to Ag super(I)-mediated enzymatic incorporation into the site opposite cytosine in the template was shown to be dATP>dTTPdCTP. Furthermore, two kinds of metal ions, Ag super(I) and Hg super(II), selectively mediate the incorporation of thymidine 5'-triphosphate into sites opposite cytosine and thymine in the template, respectively. In other words, the regulated incorporation of different metal ions into programmed sites in the duplex by DNA polymerase was successfully achieved. (Quick) silver DNA: Hg super(II) and Ag super(I) ions were found to specifically trigger DNApolymerase catalyzed primer extension in the absence of a Watson-Crick matched nucleotide through the formation of T-Hg super(II)-T and newly discovered C-Ag super(I)-T base pairs. The strict base recognition by the metal ions enabled regulated incorporation of the two different metal ions into programmed sites in duplex DNA. Metal-mediated base pairs formed by the coordination of metal ions to natural or artificial bases impart unique chemical and physical properties to nucleic acids and have attracted considerable interest in the field of nanodevices. AgI ions were found to mediate DNA polymerase catalyzed primer extension through the formation of a C-AgI-T base pair, as well as the previously reported C-AgI-A base pair. The comparative susceptibility of dNTPs to AgI-mediated enzymatic incorporation into the site opposite cytosine in the template was shown to be dATP>dTTPdCTP. Furthermore, two kinds of metal ions, AgI and HgII, selectively mediate the incorporation of thymidine 5'-triphosphate into sites opposite cytosine and thymine in the template, respectively. In other words, the regulated incorporation of different metal ions into programmed sites in the duplex by DNA polymerase was successfully achieved.
Author	Funai, Tatsuya Kiriu, Risa Wada, Shun-ichi Nakagawa, Osamu Nakamura, Junko Miyazaki, Yuki Urata, Hidehito Ono, Akira
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BackLink	https://www.ncbi.nlm.nih.gov/pubmed/24719384$$D View this record in MEDLINE/PubMed
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Copyright	2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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Keywords	silver DNA polymerase DNA structures mercury metal-mediated base pairing
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Notes	Ministry of Education, Science, Sports and Technology (Japan) ArticleID:ANIE201311235 This work was supported in part by a Grant-in-Aid for Scientific Research (C) (24550199) (for H.U.) and Scientific Research (A) (24245037) (for A.O.) from the Ministry of Education, Science, Sports and Technology (Japan). istex:0894F76B89775811F164C48681A5934224028E1C ark:/67375/WNG-VGGSHK2V-1 This work was supported in part by a Grant‐in‐Aid for Scientific Research (C) (24550199) (for H.U.) and Scientific Research (A) (24245037) (for A.O.) from the Ministry of Education, Science, Sports and Technology (Japan). ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
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Snippet	Metal‐mediated base pairs formed by the coordination of metal ions to natural or artificial bases impart unique chemical and physical properties to nucleic... Metal-mediated base pairs formed by the coordination of metal ions to natural or artificial bases impart unique chemical and physical properties to nucleic... Abstract Metal‐mediated base pairs formed by the coordination of metal ions to natural or artificial bases impart unique chemical and physical properties to...
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SubjectTerms	Biocatalysis Deoxyribonucleic acid DNA DNA - chemistry DNA - metabolism DNA polymerase DNA structures DNA-Directed DNA Polymerase - chemistry DNA-Directed DNA Polymerase - metabolism Formations Genes Incorporation Ions - chemistry mercury Metal ions metal-mediated base pairing Metals - chemistry Metals - metabolism Nucleic Acid Conformation Nucleic acids Nucleotides Physical properties Recognition Silver
Title	Regulated Incorporation of Two Different Metal Ions into Programmed Sites in a Duplex by DNA Polymerase Catalyzed Primer Extension
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